Species : |
Mycobacterium Tuberculosis |
Source : |
E.coli |
Tag : |
Non |
Description : |
DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. |
Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : |
The DnaK protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium phosphate buffer pH 7.4, 130mM sodium chloride and 2.5mM KCl. |
Solubility : |
It is recommended to reconstitute the lyophilized DnaK in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability : |
Lyophilized DnaK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DnaK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Pathways : |
RNA degradation |