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Recombinant Human Guanylate Cyclase Activator 2B (Uroguanylin)

Cat. No.: GUCA2B-127H
Product Overview: Recombinant Human Prouroguanylin produced inE.coliis 10 kDa protein containing 88 amino acid residues of the human prouroguanylin and 2 additional amino acid residues. Three-step procedure using IMAC and size exclusion chromatography before and after refolding.
Description: Prouroguanylin is a 112-amino-acid prohormone precursor of uroguanylin-mature biologically active 16-amino-acid peptide cleaved from its C-terminus. Guanylin binds to receptor-guanylate cyclases (CG-C) resulting in increased intracellular cGMP levels leading to CFTCR (Cystic Fibrosis Transmembrane Conductance Regulator) activation and subsequent rise in fluid and electrolyte secretion into the lumen. Prouroguanyline knock-out mice showed impaired renal excretion of external NaCl leading to elevated blood pressure independent of the level of diatary salt intake. Prouroguanylin is the predominant circulating molecular form found in circulation and in biological fluids.
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Form: Sterile filtered and lyophilized from 0.5 mg/ml in 50mM Phosphate buffer pH-7.4.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Specificity: The recombinant sequence is 100% homologous to the native human sequence.
Applications: Western blotting, ELISA.
Solubility: Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
Stability: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated thaw-freeze cycles and store at -80°C. Reconstituted protein can be stored at 4°C for a limited period of time. The lyophilized protein remains stable until the expiry date when stored at -20°C.
Gene Name: GUCA2B guanylate cyclase activator 2B (uroguanylin) [ Homo sapiens ]
Synonyms: GUCA2B; guanylate cyclase activator 2B (uroguanylin); UGN; GCAP-II; guanylate cyclase activator 2B; uroguanylin; OTTHUMP00000007927; uroguanylin; Guanylate cyclase C-activating peptide 2; Guanylate cyclase C-activating peptide II
Gene ID: 2981
mRNA Refseq: NM_007102
Protein Refseq: NP_009033
MIM: 601271
UniProt ID: Q16661
Chromosome Location: 1p34-p33
Function: calcium sensitive guanylate cyclase activator activity; enzyme activator activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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