- Soybean(2)
Recombinant Soy Bean P34 Protein, GST-tagged
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Cat. No. : | P34 Protein-193S |
Product Overview : | Recombinant Soybean P34 Protein epitopes expressed inE.Colicontains Soybean (Glycine max) P34 Protein epitopes 214-261, 351-379 amino acids. The protein is fused to a GST tag at N-terminus. |
Description : | The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2–3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a 32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons. |
Source : | E.Coli. |
Physical Appearance : | Use as an antigen in ELISA and Western Blots. |
Formulation : | 50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol. |
Stability : | Five years frozen. One month in solution at room temperature. |
Purity : | Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC. |
Storage : | Protein is shipped at ambient temperature. Upon arrival, Store at -20°C |
Gene Name : | LOC548062 34 kDa maturing seed vacuolar thiol protease precursor [ Glycine max ] |
Synonyms : | P34 Protein; 34 kDa maturing seed vacuolar thiol protease precursor; P34 probable thiol protease;EC=3.4.22.- |
Gene ID : | 548062 |
UniProt ID : | P22895 |
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