| Cat. No. : |
P34 Protein-194S |
| Product Overview : |
Recombinant Soy bean P34 Protein epitopes expressed inE.Colicontains Soy bean P34 Protein epitopes 214-261, 351-379 amino acids. The protein is fused to a His tag at N-terminus. |
| Description : |
The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2–3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a 32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons. |
| Source : |
E.Coli. |
| Method : |
Purified by proprietary chromatographic technique. |
| Physical Appearance : |
Use as an antigen in ELISA and Western Blots. |
| Formulation : |
50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol. |
| Stability : |
Five years frozen. One month in solution at room temperature. |
| Purity : |
Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC. |
| Storage : |
Protein is shipped at ambient temperature. Upon arrival, Store at -20°C |
