Recombinant Soy Bean P34 Protein, His-tagged


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Recombinant Soy Bean P34 Protein, His-tagged

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Cat. No. : P34 Protein-194S
Product Overview : Recombinant Soy bean P34 Protein epitopes expressed inE.Colicontains Soy bean P34 Protein epitopes 214-261, 351-379 amino acids. The protein is fused to a His tag at N-terminus.
Description : The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2–3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a 32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons.
Source : E.Coli.
Method : Purified by proprietary chromatographic technique.
Physical Appearance : Use as an antigen in ELISA and Western Blots.
Formulation : 50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.
Stability : Five years frozen. One month in solution at room temperature.
Purity : Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
Storage : Protein is shipped at ambient temperature. Upon arrival, Store at -20°C
Gene Name : LOC548062 34 kDa maturing seed vacuolar thiol protease precursor [ Glycine max ]
Synonyms : LOC548062; 34 kDa maturing seed vacuolar thiol protease precursor; P34 probable thiol protease;EC=3.4.22.-
Gene ID : 548062
UniProt ID : P22895

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