Recombinant Soy Bean P34 Protein, His-tagged

• Cat.No.: P34Protein-194S
• Product Overview: Recombinant Soy bean P34 Protein epitopes expressed inE.Colicontains Soy bean P34 Protein epitopes 214-261, 351-379 amino acids. The protein is fused to a His tag at N-terminus.

Specification

• Species: Soybean
• Source: E.coli
• Tag: His
• Protein Length: 214-261;351-379 a.a.
• Description: The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2–3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a 32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons.
• Method: Purified by proprietary chromatographic technique.
• Physical Appearance: Use as an antigen in ELISA and Western Blots.
• Formulation: 50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.
• Stability: Five years frozen. One month in solution at room temperature.
• Purity: Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
• Storage: Protein is shipped at ambient temperature. Upon arrival, Store at -20°C

Gene Information

• Gene Name: LOC548062 34 kDa maturing seed vacuolar thiol protease precursor [ Glycine max ]
• Synonyms: LOC548062; 34 kDa maturing seed vacuolar thiol protease precursor; P34 probable thiol protease;EC=3.4.22.-
• Gene ID: 548062
• UniProt ID: P22895