||Recombinant humanAurora Kinase B, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 41.4 kDa (364aa).
||Aurora Kinase B is a member of a family of mitotic serine/threonine kinases. Aurora Kinase B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora Kinase B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of Aurora Kinase B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation, while overexpression in many kinds of cancers.
|Amino Acid Sequence:
||MGSSHHHHHH SSGLVPRGSH MAQKENSYPW PYGRQTAPSG LSTLPQRVLR KEPVTPSALV LMSRSNVQPT AAPGQKVMEN SSGTPDILTR HFTIDDFEIG RPLGKGKFGN VYLAREKKSH FIVALKVLFK SQIEKEGVEH QLRREIEIQA HLHHPNILRL YNYFYDRRRI YLILEYAPRG ELYKELQKSC TFDEQRTATI MEELADALMY CHGKKVIHRD IKPENLLLGL KGELKIADFG WSVHAPSLRR KTMCGTLDYL PPEMIEGRMH NEKVDLWCIG VLCYELLVGN PPFESASHNE TYRRIVKVDL KFPASVPMGA QDLISKLLRH NPSERLPLAQ VSAHPWVRAN SRRVLPPSAL QSVA
||Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 0.5mM DTT, 20% glycerol, 0.1mM EDTA, 0.1mM EGTA, 0.1M NaCl, 0.1mM PMSF.
||> 90% by SDS – PAGE.
||0.5 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.