Recombinant Human Cell Division Cycle 42, T7-tagged

• Cat.No.: CDC42-1394H
• Product Overview: Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 22.4 kDa (203aa).

Specification

• Cat. No.: CDC42-1394H
• Description: Cell division cycle 42 isoform 1, also known as CDC42,is a smallGTPaseof the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs.
• Source: E.coli.
• Amino Acid Sequence: MASMTGGQQM GRGSHMQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC
• Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA.
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CDC42 cell division cycle 42 (GTP binding protein, 25kDa) [ Homo sapiens ]
• Synonyms: CDC42; cell division cycle 42 (GTP binding protein, 25kDa); G25K; CDC42Hs; cell division cycle 42; OTTHUMP00000002834; GTP-binding protein, 25kD; growth-regulating protein; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); Cell division control protein 42 homolog; G25K GTP-binding protein; OTTHUMP00000002926; cell division cycle 42 (GTP binding protein, 25kD); cell division cycle 42 (GTP-binding protein, 25kD)
• Gene ID: 998
• mRNA Refseq: NM_001039802
• Protein Refseq: NP_001034891
• MIM: 116952
• UniProt ID: P60953
• Chromosome Location: 1p36.1
• Pathway: Adherens junction; Axon guidance; Chemokine signaling pathway; Endocytosis; Epithelial cell signaling in Helicobacter pylori infection; Fc gamma R-mediated phagocytosis; Focal adhesion; GnRH signaling pathway; Leukocyte transendothelial migration; MAPK signaling pathway; Neurotrophin signaling pathway; Pancreatic cancer; Pathogenic Escherichia coli infection; Pathways in cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; T cell receptor signaling pathway; Tight junction; VEGF signaling pathway; Axon guidance; Signaling by EGFR; Signaling by Rho GTPases
• Function: GTP binding; GTPase activity; nucleotide binding; protein binding