Recombinant Human Histidyl-tRNA Synthetase, His-tagged

• Cat.No.: HARS-369H
• Product Overview: Recombinant Human Histidyl-tRNA Synthetase produced inbaculovirusis a single, glycosylated, polypeptide chain having a molecular mass of 58.3 kDa. The Histidyl-tRNA Synthetase is fused to 6xHis Tag and purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
• Physical Appearance: Sterile Filtered clear solution.
• Formulation: The protein solution contains 20mM HEPES, 250mM sodium chloride 0.1% and 20% Glycerol, (pH 7.5).
• Western Blot: Strongly reactive with human anti Histidyl-tRNA Synthetase antisera.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Lyophilized Histidyl-tRNA Synthetase although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: HARS histidyl-tRNA synthetase [ Homo sapiens ]
• Synonyms: HARS; histidyl-tRNA synthetase; HRS; FLJ20491; histidine tRNA ligase 1, cytoplasmic; EC 6.1.1.21; HisRS; histidine-tRNA ligase
• Gene ID: 3035
• mRNA Refseq: NM_002109
• Protein Refseq: NP_002100
• MIM: 142810
• UniProt ID: P12081
• Chromosome Location: 5q31.3
• Pathway: Aminoacyl-tRNA biosynthesis; Gene Expression
• Function: ATP binding; histidine-tRNA ligase activity; histidine-tRNA ligase activity; nucleotide binding