||Recombinant Human Histidyl-tRNA Synthetase produced inbaculovirusis a single, glycosylated, polypeptide chain having a molecular mass of 58.3 kDa. The Histidyl-tRNA Synthetase is fused to 6xHis Tag and purified by proprietary chromatographic techniques.
||Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
||Sf9 Insect Cells.
||Sterile Filtered clear solution.
||The protein solution contains 20mM HEPES, 250mM sodium chloride 0.1% and 20% Glycerol, (pH 7.5).
||Strongly reactive with human anti Histidyl-tRNA Synthetase antisera.
||Greater than 90% as determined by SDS-PAGE.
||Lyophilized Histidyl-tRNA Synthetase although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.