Recombinant Human BMF, T7-tagged

• Cat.No.: BMF-1350H
• Product Overview: Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: T7
• Protein Length: 1-129aa
• Description: The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing, 1mM DTT, 10% glycerol
• Molecular Mass: 15.6 kDa (144aa), confirmed by MALDI-TOF.
• AA Sequence: MASMTGGQQM GRGSHMEPSQ CVEELEDDVF QPEDGEPVTQ PGSLLSADLF AQSLLDCPLS RLQLFPLTHC CGPGLRPTSQ EDKATQTLSP ASPSQGVMLP CGVTEEPQRL FYAPAEPKSC VVADPPLPAQ PCFEWRREQE RGRP
• Endotoxin: < 1.0 eu per 1 microgram of protein (determined by lal
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: BMF Bcl2 modifying factor [ Homo sapiens ]
• Official Symbol: BMF
• Synonyms: BMF; Bcl2 modifying factor; FLJ00065; Bcl-2-modifying factor
• Gene ID: 90427
• mRNA Refseq: NM_001003943
• Protein Refseq: NP_001003943
• MIM: 606266
• UniProt ID: Q96LC9
• Chromosome Location: 15q14
• Pathway: Activation of BH3-only proteins; Activation of BMF and translocation to mitochondria; Androgen receptor signaling pathway
• Function: protein binding