||Recombinant human Full-length GCK was expressed by baculovirus inSf9 cellsusing an N-terminal GST tag. MW=125kDa.
||GCK is a serine/threonine protein kinase that is activated by TNFα and interacts with the TNF receptor-associated factor 2 (TRAF2). GCK is upstream of MAP kinases and particularly activates the SAPK pathway. Although GCK is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. A mouse protein Rab8ip that has high homology to GCK can interact with the vesicular transport protein Rab8. Based on its interaction with Rab8, it is postulated that Rab8ip/GCK may modulate secretion in response to stress stimuli.
||Sf9 insect cells using baculovirus.
||Kinase Assay, Western Blot.
|Storage And Stability:
||Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.