Recombinant Human AMPK (A1/B1/G2), His-tagged, Active

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Recombinant Human AMPK (A1/B1/G2), His-tagged, Active

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Specification Gene Information
Cat. No. : AMPK (A1/B1/G2)-242H
Product Overview : Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus inSf9 insect cellsusing a C-terminal His tags. MW = 68kDa (A1), 38kDa (B1), 65kDa (G2).
Description : AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γsubunit allosterically activates the complex. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signaling pathways in response to adiponectin, leptin and CAMKKβ.
Source : Sf9 insect cells using baculovirus.
Sequence : Full-lengt h.
Applications : Kinase Assay, Western Blot.
Storage And Stability : Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Gene Name : PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit [ Homo sapiens ],PRKAB1 protein kinase, AMP-activated, beta 1 non-catalytic subunit [ Homo sapiens ],PRKAG2 protein kinase, AMP-activated, gamma 2 non-catalytic subunit [ Homo sapiens ]
Synonyms : protein kinase, AMP-activated, alpha 1 catalytic subunit; AMPK; AMPKa1; MGC33776; MGC57364; PRKAA1; AMPK alpha 1; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; 5"-AMP-activated protein kinase, catalytic alpha-1 chain; AMPK1EC; 2.7.11.1; AMPK alpha-1 chain, protein kinase, AMP-activated, beta 1 non-catalytic subunit; AMPK; HAMPKb; MGC17785; PRKAB1; AMPK beta 1; AMPK beta -1 chain; AMP-activated protein kinase beta subunit; 5"-AMP-activated protein kinase beta-1 subunit; protein kinase, AMP-activated, noncatalytic, beta-1; AMPKb; protein kinase, AMP-activated, beta 1 non-catalytic subunit, protein kinase, AMP-activated, gamma 2 non-catalytic subunit; AAKG; CMH6; WPWS; AAKG2; H91620p; PRKAG2; AMP-activated protein kinase gamma2 subunit; AMPK gamma2; AMPK gamma-2 chain; protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Gene ID : 5562,5564,51422
mRNA Refseq : NM_006251,NM_006253,NM_001040633
Protein Refseq : NP_006242,NP_006244,NP_001035723
MIM : 602739,602740,602743
UniProt ID : Q13131, Q9Y478, Q9UGJ0
Chromosome Location : 5p12, 12q24.1, 7q36.1
Pathway : Adipocytokine signaling pathway; Hypertrophic cardiomyopathy (HCM); Insulin signaling pathway; Regulation of autophagy; mTOR signaling pathway, Adipocytokine signaling pathway; Hypertrophic cardiomyopathy (HCM); Insulin signaling pathway, Adipocytokine signaling pathway; Hypertrophic cardiomyopathy (HCM); Insulin signaling pathway
Function : ATP binding; cAMP-dependent protein kinase activity; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity, protein binding; protein kinase binding, ADP binding; AMP binding; AMP-activated protein kinase activity; ATP binding; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; phosphorylase kinase regulator activity; protein kinase activator activity; protein kinase binding

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