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Recombinant Human MMP9, Catalytic Domain, T7-tagged

Cat.No. : MMP9-774H
Product Overview : Recombinant human Metalloproteinase-9 (MMP-9, 92 kDa type IV collagenase, 92 kDa gelatinase, Gelatinase B, GELB) catalytic domain with fibronectins domains cloned from human cDNA, was expressed inE. coli.The recombinant enzyme consists of the catalytic domain of human MMP-9 (residues 112-445) with a N-term T7 tag. MW= 37.7kDa.
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  • Gene Information
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Cat. No. : MMP9-774H
Description : Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Source : E. coli.
Purity : > 95% by SDS-PAGE. In an SDS-PAGE gel, the enzyme runs as a single band migrating between 35 and 45 kDa.
Supplied As : 0.15mg/ml in 50mM Tris pH 7.2, 5mM CaCl2, 0.1mM ZnCl2, 0.3M NaCl, 0.5M Acetohydroxamic Acid (AHA), 10% glycerol, Brij35 0.05%. The concentration is calculated from the absorbance at 280nm (ε280 = 67100 M-1 cm-1calculated).
Specific Activity : >10U/μg. 1U=100pmol/min at 25ºC using a colorimetric assay with thiopeptolide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol).
Usage : Study enzyme kinetics, cleavage of target substrates and screen for inhibitors.
Storage : -80℃. It is recommended that thawing and dilution of the enzyme be done in ice and within as short a time as possible before start of the assay. After initial defrost, aliquot product into individual tubes and refreeze at -80℃. Avoid repeated freeze/defrost cycles.
Gene Name : MMP9 matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) [ Homo sapiens ]
Synonyms : MMP9; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); GELB; CLG4B; MMP-9; MANDP2; matrix metalloproteinase 9; type V collagenase; macrophage gelatinase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); EC 3.4.24.35; Matrix metalloproteinase-9; 92 kDa type IV collagenase; 92 kDa gelatinase; Gelatinase B; GELB; 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; OTTHUMP00000031674; type V collagenase
Gene ID : 4318
mRNA Refseq : NM_004994
Protein Refseq : NP_004985
MIM : 120361
UniProt ID : P14780
Chromosome Location : 20q12-q13
Pathway : Bladder cancer; Leukocyte transendothelial migration; Pathways in cancer
Function : calcium ion binding; collagen binding; metalloendopeptidase activity; peptidase activity; zinc ion binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What are the consequences of dysregulated MMP9 expression in cancer progression? 02/13/2022

Dysregulated MMP9 expression is associated with cancer invasion and metastasis, as it enables tumor cells to degrade the extracellular matrix and invade surrounding tissues.

What is the impact of MMP9 polymorphisms on enzyme activity? 09/07/2021

Polymorphisms in the MMP9 gene can influence enzyme activity, potentially affecting the individual's susceptibility to certain diseases and the progression of pathological conditions.

Can you explain the role of MMP9 in tissue remodeling at the molecular level? 01/17/2020

MMP9 facilitates tissue remodeling by cleaving structural proteins in the extracellular matrix, such as collagen and gelatin, leading to changes in tissue architecture.

What is the role of MMP9 in the breakdown of the blood-brain barrier during neurological disorders? 11/14/2019

MMP9 contributes to the breakdown of the blood-brain barrier by degrading tight junction proteins, potentially exacerbating neurological disorders and neuroinflammation.

How does MMP9 contribute to angiogenesis at the molecular level? 06/02/2018

MMP9 plays a role in angiogenesis by promoting the release and activation of growth factors sequestered in the extracellular matrix, facilitating the formation of new blood vessels.

Customer Reviews (3)

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Reviews
05/03/2020

    The superior quality of this recombined protein has significantly enhanced the precision of our research outcomes.

    08/15/2019

      Fast shipping! Ordered this product, and it arrived well-packaged and ready for use in our experiments.

      01/21/2018

        Customer service went above and beyond to accommodate our specific needs, ensuring a smooth purchasing experience.

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