Recombinant Human AHCY, His-tagged

• Cat.No.: AHCY-31342TH
• Product Overview: Recombinant human AHCY protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family. It catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
• Molecular Mass: 49.8 kDa (452aa) confirmed by MALDI-TOF
• Protein length: 1-432aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSDKLPYKVA DIGLAAWGRK ALDIAENEMP GLMRMRERYS ASKPLKGARI AGCLHMTVET AVLIETLVTLGAEVQWSSCN IFSTQDHAAA AIAKAGIPVY AWKGETDEEY LWCIEQTLYF KDGPLNMILD DGGDLTNLIH TKYPQLLPGI RGISEETTTGVHNLYKMMAN GILKVPAINV NDSVTKSKFD NLYGCRESLI DGIKRATDVM IAGKVAVVAG YGDVGKGCAQ ALRGFGARVI ITEIDPINALQAAMEGYEVT TMDEACQEGN IFVTTTGCID IILGRHFEQM KDDAIVCNIG HFDVEIDVKW LNENAVEKVN IKPQVDRYRL KNGRRIILLAEGRLVNLGCA MGHPSFVMSN SFTNQVMAQI ELWTHPDKYP VGVHFLPKKL DEAVAEAHLG KLNVKLTKLT EKQAQYLGMS CDGPFKPDHYRY
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)
• Publication:
  H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase. (2015)

Gene Information

• Gene Name: AHCY adenosylhomocysteinase [ Homo sapiens (human) ]
• Official Symbol: AHCY
• Synonyms: AHCY; adenosylhomocysteinase; S adenosylhomocysteine hydrolase; SAHH; adoHcyase; S-adenosylhomocysteine hydrolase; S-adenosyl-L-homocysteine hydrolase; NP_000678.1; EC 3.3.1.1; NP_001155238.1
• Gene ID: 191
• mRNA Refseq: NM_000687
• Protein Refseq: NP_000678
• MIM: 180960
• UniProt ID: P23526
• Chromosome Location: 20q11.22
• Pathway: Biological oxidations; deficiency (HMAHCHD); Defective GGT1 causes Glutathionuria (GLUTH)
• Function: adenosylhomocysteinase activity; adenyl nucleotide binding; identical protein binding