Recombinant Human ALDH1A1 protein

• Cat.No.: ALDH1A1-27035TH
• Product Overview: Recombinant Human ALDH1A1(1-501aa) was expressed in E. coli.

Specification

• Description: Aldehyde dehydrogenase 1A1 (ALDH1A1), also known as retinal dehydrogenase 1, is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea. (Retinal + NAD+ + H2O = retinoate + NADH).
• Source: E. coli
• Species: Human
• Form: Liquid. In 50mM Tris-HCl buffer (pH7.5) containing 10% glycerol
• Molecular Mass: 54.8 kDa (501aa), confirmed by MALDI-TOF.
• AA Sequence: MSSSGTPDLP VLLTDLKIQY TKIFINNEWH DSVSGKKFPV FNPATEEELC QVEEGDKEDV DKAVKAARQA FQIGSPWRTM DASERGRLLYKLADLIERDR LLLATMESMN GGKLYSNAYL NDLAGCIKTL RYCAGWADKI QGRTIPIDGN FFTYTRHEPI GVCGQIIPWN FPLVMLIWKIGPALSCGNTV VVKPAEQTPL TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDID KVAFTGSTEV GKLIKEAAGK SNLKRVTLELGGKSPCIVLA DADLDNAVEF AHHGVFYHQG QCCIAASRIF VEESIYDEFV RRSVERAKKY ILGNPLTPGV TQGPQIDKEQ YDKILDLIESGKKEGAKLEC GGGPWGNKGY FVQPTVFSNV TDEMRIAKEE IFGPVQQIMK FKSLDDVIKR ANNTFYGLSA GVFTKDIDKA ITISSALQAGTVWVNCYGVV SAQCPFGGFK MSGNGRELGE YGFHEYTEVK TVTVKISQKN S
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ALDH1A1 aldehyde dehydrogenase 1 family, member A1 [ Homo sapiens ]
• Official Symbol: ALDH1A1
• Synonyms: ALDH1A1; aldehyde dehydrogenase 1 family, member A1; ALDH1, PUMB1; retinal dehydrogenase 1; RALDH1; retinaldehyde dehydrogenase 1; ALHDII; RALDH 1; ALDH class 1; acetaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic; ALDC; ALDH1; PUMB1; ALDH11; ALDH-E1; MGC2318
• Gene ID: 216
• mRNA Refseq: NM_000689
• Protein Refseq: NP_000680
• MIM: 100640
• UniProt ID: P00352
• Chromosome Location: 9q21.13
• Pathway: Biological oxidations, organism-specific biosystem; Ethanol oxidation, organism-specific biosystem; Fatty Acid Omega Oxidation, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Phase 1 - Functionalization of compounds, organism-specific biosystem; Retinol metabolism, organism-specific biosystem
• Function: Ras GTPase activator activity; aldehyde dehydrogenase (NAD) activity; androgen binding; oxidoreductase activity; retinal dehydrogenase activity

Reviews

08/05/2020

The attention to detail in quality assurance is commendable. It's a lab favorite for a reason.

03/12/2019

Great for assays and experiments. Dependable results make it a lab favorite.

11/23/2018

A lab staple! Quality is never compromised, and the shipping is always reliable.

Q&As

How does ALDH1A1 contribute to cellular detoxification by metabolizing endogenous and exogenous aldehydes?

ALDH1A1 contributes to cellular detoxification by metabolizing endogenous and exogenous aldehydes, reducing their potential cytotoxic effects.

What are the structural features of ALDH1A1 that are crucial for its catalytic activity, and how do substrate binding and cofactor interaction occur?

ALDH1A1's catalytic activity depends on its conserved structural features. Substrate binding involves the aldehyde-binding pocket, and cofactor interaction occurs through the binding of NAD+.

How does ALDH1A1 participate in retinoid homeostasis, and what are the consequences of its dysregulation in diseases like vitamin A deficiency or excess?

ALDH1A1 contributes to retinoid homeostasis by regulating the levels of active retinoic acid. Dysregulation may lead to diseases associated with vitamin A deficiency or excess.

What is the enzymatic function of ALDH1A1, and how does it catalyze the conversion of aldehydes?

ALDH1A1 is an aldehyde dehydrogenase enzyme responsible for catalyzing the oxidation of retinaldehyde to retinoic acid, a critical step in the synthesis of retinoids.

Can you explain the role of ALDH1A1 in retinoic acid signaling and its impact on cellular processes, including gene expression and differentiation?

ALDH1A1 plays a key role in retinoic acid signaling by generating active retinoic acid. This signaling pathway influences gene expression and cellular differentiation in various tissues.