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Recombinant Human AURKB, His-tagged

Cat.No. : AURKB-27422TH
Product Overview : Recombinant fragment, corresponding to amino acids 2-344 of Human Aurora B with a proprietary enzyme and His tag
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Description : Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al.
Conjugation : HIS
Tissue specificity : High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Biological activity : 12 U/ug. One unit is defined as the amount of enzyme that willphosphorylate 1 pmol of Ser/Thr 1 peptide substarte per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Naorthovanadate, 0.5 mM ATP, 4 uM au
Form : Liquid
Storage buffer : Preservative: NoneConstituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0
Storage : Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Sequences of amino acids : AQKENSYPW PYGRQTAPSG LSTLPQRVLR KEPVTPSALV LMSRSNVQPT AAPGQKVMEN SSGTPDILTR HFTIDDFEIG RPLGKGKFGN VYLAREKKSH FIVALKVLFK SQIEKEGVEH QLRREIEIQA HLHHPNILRL YNYFYDRRRI YLILEYAPRG ELYKELQKSC TFDEQRTATI MEELADALMY CHGKKVIHRD IKPENLLLGL KGELKIADFG WSVHAPSLRR KTMCGTLDYL PPEMIEGRMH NEKVDLWCIG VLCYELLVGN PPFESASHNE TYRRIVKVDL KFPASVPTGA QDLISKLLRH NPSERLPLAQ VSAHPWVRAN SRRVLPPSAL QSVA
Sequence Similarities : Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.Contains 1 protein kinase domain.
Gene Name : AURKB aurora kinase B [ Homo sapiens ]
Official Symbol : AURKB
Synonyms : AURKB; aurora kinase B; serine/threonine kinase 12 , STK12; Aik2; AIM 1; ARK2; AurB; aurora 1; aurora B; IPL1; PPP1R48; protein phosphatase 1; regulatory subunit 48; STK5;
Gene ID : 9212
mRNA Refseq : NM_004217
Protein Refseq : NP_004208
MIM : 604970
Uniprot ID : Q96GD4
Chromosome Location : 17p13.1
Pathway : APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Aurora C signaling, organism-specific biosystem;
Function : ATP binding; metal ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What are the interactions between AURKB protein and cell cycle regulatory proteins? 12/03/2022

AURKB protein interacts with a variety of cell cycle regulatory proteins, including Cdk1, INCENP, Survivin, etc., to regulate the progression of cell mitosis.

What are the intracellular substrates of AURKB protein? 09/09/2022

The substrates of AURKB include chromosome-associated proteins, cytoskeletal proteins and other cell cycle regulatory proteins. Phosphorylation modification of these substrates regulates chromosome segregation and cell division during mitosis.

What is the cellular localization of AURKB protein? 06/30/2021

AURKB protein is mainly located in the nucleus and cytoplasm and participates in the regulation of cell mitosis.

What is the expression pattern of AURKB gene? 08/11/2020

The expression of the AURKB gene is regulated by cell cycle regulators and other transcription factors and is mainly expressed in the mitotic phase of cells.

How does AURKB protein activity work? 03/14/2020

The activity of AURKB protein is regulated by a variety of factors, including phosphorylation modification, protein interaction and cell cycle regulation.

At what stage of cell mitosis does AURKB protein reach its peak expression? 04/08/2019

The protein reaches its peak expression during early and metaphase of cell mitosis and is closely associated with chromosome segregation and cell division.

Customer Reviews (3)

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Reviews
12/28/2021

    AURKB's exceptional repeatability allows for its reliable use in a range of applications, making it a top choice for researchers.

    08/31/2021

      Very pure and can be used with confidence.

      02/03/2019

        The quality is very high and very reliable.

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