Recombinant Human BLNK Protein, His-tagged

• Cat.No.: BLNK-26279TH
• Product Overview: Recombinant Human BLNK(1-456aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: BLNK, also known as B-cell linker, is essential for normal B-cell development. This protein has been shown to associate with the effector proteins GRB2, Vav, NCK and PLC gamma following activation of the B cell receptor. It is phosphorylated by the Syk tyrosine kinase, which in turn permits activation of downstream effector proteins including GRB2 and PLC gamma.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
• Molecular Mass: 52.6 kDa (476aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• Protein length: 1-456aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSHDYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQMDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EQQWSDDFDSKPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGKKPTTPLKTTP VASQQNASSV CEEKPIPAERHRGSSHRQEAVQSPV FPPAQKQIHQKPIPLPRFTEGGNPT VDGPLPSFSSNSTISEQEAGVLCKP WYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQHSPLVLIDSQ NNTKDSTRLK YAVKVS
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: BLNK B-cell linker [ Homo sapiens ]
• Official Symbol: BLNK
• Synonyms: BLNK; B-cell linker; B-cell linker protein; B cell adaptor containing SH2 domain; B cell activation; B cell adapter containing a SH2 domain protein; BASH; bca; BLNK s; Ly57; SLP 65; SLP65; Src homology [SH2] domain containing leukocyte protein of 65 kD; B-cell activation; B cell linker protein; cytoplasmic adapter protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Src homology 2 domain-containing leukocyte protein of 65 kDa; Src homology [SH2] domain-containing leukocyte protein of 65 kD; AGM4; LY57; BLNK-S; SLP-65; MGC111051
• Gene ID: 29760
• mRNA Refseq: NM_001114094
• Protein Refseq: NP_001107566
• MIM: 604515
• UniProt ID: Q8WV28