Recombinant Human BRAF

• Cat.No.: BRAF-26567TH
• Product Overview: Recombinant fragment, corresponding to amino acids 432-725 of Human B Raf coexpressed with P50.MW is 36 kDa.

Specification

• Description: This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
• Tissue specificity: Brain and testis.
• Biological activity: Activity was tested using MEK1 as substrate. Assay buffer: 50 mMHEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1mM ATP
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 40% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 150mM Sodium chloride, pH 8.0
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: SEDRNRMKT LGRRDSSDDW EIPDGQITVG QRIGSGSFGT VYKGKWHGDV AVKMLNVTAP TPQQLQAFKN EVGVLRKTRH VNILLFMGYS TKPQLAIVTQ WCEGSSLYHH LHIIETKFEM IKLIDIARQT AQGMDYLHAK SIIHRDLKSN NIFLHEDLTV KIGDFGLATV KSRWSGSHQF EQLSGSILWM APEVIRMQDK NPYSFQSDVY AFGIVLYELM TGQLPYSNIN NRDQIIFMVG RGYLSPDLSK VRSNCPKAMK RLMAECLKKK RDERPLFPQI LASIELLARS LPKIH
• Sequence Similarities: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.Contains 1 RBD (Ras-binding) domain.

Gene Information

• Gene Name: BRAF v-raf murine sarcoma viral oncogene homolog B1 [ Homo sapiens ]
• Official Symbol: BRAF
• Synonyms: BRAF; v-raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf; BRAF1
• Gene ID: 673
• mRNA Refseq: NM_004333
• Protein Refseq: NP_004324
• MIM: 164757
• Uniprot ID: P15056
• Chromosome Location: 7q34
• Pathway: ARMS-mediated activation, organism-specific biosystem; Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem
• Function: ATP binding; MAP kinase kinase kinase activity; metal ion binding; mitogen-activated protein kinase kinase binding; nucleotide binding