Recombinant Human CBR1 protein

• Cat.No.: CBR1-26326TH
• Product Overview: Recombinant Human CBR1(1-277aa) was expressed in E. coli.

Specification

• Description: CBR1(Carbonyl reductase 1) is one of several monomeric NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. CBR1 is widely distributed in human tissues. CBR1 metabolizes many toxic environmental quinones and pharmacological relevant substrates such as the anticancer doxorubicin. CBR1 can also convert prostaglandin E2 to prostaglandin F2-alpha.
• Source: E. coli
• Species: Human
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol
• Molecular Mass: 30 kDa (277aa), confirmed by MALDI-TOF.
• AA Sequence: MSSGIHVALV TGGNKGIGLA IVRDLCRLFS GDVVLTARDV TRGQAAVQQL QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLDVLVNNAGIAFKVADP TPFHIQAEVT MKTNFFGTRD VCTELLPLIK PQGRVVNVSS IMSVRALKSC SPELQQKFRS ETITEEELVG LMNKFVEDTKKGVHQKEGWP SSAYGVTKIG VTVLSRIHAR KLSEQRKGDK ILLNACCPGW VRTDMAGPKA TKSPEEGAET PVYLALLPPD AEGPHGQFVSEKRVEQW
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CBR1 carbonyl reductase 1 [ Homo sapiens ]
• Official Symbol: CBR1
• Synonyms: CBR1; carbonyl reductase 1; CBR; carbonyl reductase [NADPH] 1; SDR21C1; short chain dehydrogenase/reductase family 21C; member 1; carbonyl reductase (NADPH) 1; prostaglandin 9-ketoreductase; prostaglandin-E(2) 9-reductase; NADPH-dependent carbonyl reductase 1; 15-hydroxyprostaglandin dehydrogenase; short chain dehydrogenase/reductase family 21C, member 1; hCBR1
• Gene ID: 873
• mRNA Refseq: NM_001757
• Protein Refseq: NP_001748
• MIM: 114830
• UniProt ID: P16152
• Chromosome Location: 21q22.1
• Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism of xenobiotics by cytochrome P450, organism-specific biosystem; Metabolism of xenobiotics by cytochrome P450, conserved biosystem
• Function: 15-hydroxyprostaglandin dehydrogenase (NADP+) activity; carbonyl reductase (NADPH) activity; nucleotide binding; oxidoreductase activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; prostaglandin-E2 9-reductase activity