Recombinant Human CRK, His-tagged

• Cat.No.: CRK-27823TH
• Product Overview: Recombinant full length Human Crk p38 protein with an N terminal His-tag.

Specification

• Description: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
• Protein length: 224 amino acids
• Conjugation: HIS
• Molecular Weight: 25.000kDa
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 20mM Tris HCl, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMAGNF DSEERSSWYWGRLSRQEAVALLQGQ RHGVFLVRDSSTSPGDYVLSVSENS RVSHYIINSSGPRPPVPPSPAQPPP GVSPSRLRIGDQEFDSLPALLEFYK IHYLDTTTLIEPVSRSRQGSGVILR QEEAEYVRALFDFNGNDEEDLPFKK GDILRIRDKPEEQWWNAEDSEGKRG MIPVPYVEKYRPASASVSALIGGR
• Sequence Similarities: Belongs to the CRK family.Contains 1 SH2 domain.Contains 2 SH3 domains.

Gene Information

• Gene Name: CRK v-crk sarcoma virus CT10 oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: CRK
• Synonyms: CRK; v-crk sarcoma virus CT10 oncogene homolog (avian); v crk avian sarcoma virus CT10 oncogene homolog; adapter molecule crk
• Gene ID: 1398
• mRNA Refseq: NM_005206
• Protein Refseq: NP_005197
• MIM: 164762
• Uniprot ID: P46108
• Chromosome Location: 17p13
• Pathway: ARMS-mediated activation, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem
• Function: SH2 domain binding; SH3/SH2 adaptor activity; ephrin receptor binding; protein binding; protein phosphorylated amino acid binding