Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human DEFB4A protein

Cat.No. : DEFB4A-27476TH
Product Overview : Recombinant Human DEFB4A protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
  • Related Products
Description : Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human β-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because β-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.
Source : E.coli
Species : Human
Form : Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
Bio-activity : Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration range of 10-100 ng/ml.
Molecular Mass : Approximately 4.3 kDa, a single non-glycosylated polypeptide chain containing 41 amino acids.
Protein length : 41
AA Sequence : GIGDPVTCLKSGAICHPVFCPRRYKQIGTCGLPGTKCCKKP
Endotoxin : Less than 1 EU/µg of rHuBD-2 as determined by LAL method.
Purity : >98% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : DEFB4A
Official Symbol : DEFB4A
Synonyms : DEFB4A; defensin, beta 4A; DEFB2, DEFB4, DEFB102, defensin, beta 2 , defensin, beta 4; beta-defensin 4A; DEFB 2; HBD 2; SAP1; defensin, beta 2; defensin, beta 4; skin-antimicrobial peptide 1; BD-2; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102;
Gene ID : 1673
mRNA Refseq : NM_004942
Protein Refseq : NP_004933
MIM : 602215
UniProt ID : O15263

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (6)

Ask a question
How can the structure of DEFB4A be evaluated? 05/25/2023

DEFB4A has a typical β-defensin structure consisting of a cysteine-rich mature peptide region and a positively charged N-terminal region. This structure allows it to bind and destroy the cell membranes of pathogenic microorganisms.

Which biomolecules can be used as molecular markers for DEFB4A? 03/16/2023

Biomolecules that can be used as molecular markers for DEFB4A include its mRNA and protein expression products in tissues and cells, as well as other biomolecules (e.g., receptors, signal transduction molecules, etc.) that interact with it.

What diseases are associated with the aberrant expression of DEFB4A? 07/26/2022

The abnormal expression of DEFB4A is associated with a variety of inflammatory and infectious diseases, such as psoriasis, atopic dermatitis, chronic sinusitis, etc. In addition, the expression of DEFB4A has also been found to be associated with the occurrence and progression of certain tumors.

What are the treatment strategies for DEFB4A? 03/07/2021

Therapeutic strategies for DEFB4A include modulating its expression levels, enhancing its antimicrobial activity, and developing inhibitors targeting its signaling pathway. Treatment depends on the type and severity of the disease.

How can I detect the expression level of DEFB4A? 11/15/2020

The mRNA and protein expression levels of DEFB4A in tissues and cells can be detected by real-time PCR, immunohistochemistry, and western blotting.

What is the interaction between DEFB4A and other biomolecules? 02/01/2020

There are interactions between DEFB4A and other biomolecules. For example, it can bind to the cell membrane of pathogenic microorganisms and disrupt their integrity to exert antimicrobial effects, and it can also bind to receptors on the surface of epithelial cells to activate signal transduction pathways and regulate cellular immune responses.

Customer Reviews (3)

Write a review
Reviews
06/28/2023

    The structure is very stable and not easy to deteriorate.

    06/01/2023

      The cost is low and the activity is strong, which greatly saves our scientific research costs.

      04/09/2020

        The quality of the DEFB4A is very high, which makes my experiments more smooth and accurate.

        Ask a Question for All DEFB4A Products

        Required fields are marked with *

        My Review for All DEFB4A Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends