Recombinant Human DOT1L

• Cat.No.: DOT1L-27742TH
• Product Overview: Recombinant fragment, corresponding to amino acids 2-416 of Human KMT4 / Dot1L with N terminal proprietary tag, 73 kDa.

Specification

• Description: The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes.
• Source: E. coli
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 7.5
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Gene Information

• Gene Name: DOT1L DOT1-like, histone H3 methyltransferase (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: DOT1L
• Synonyms: DOT1L; DOT1-like, histone H3 methyltransferase (S. cerevisiae); histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1; histone methyltransferase DOT1L; KIAA1814; KMT4
• Gene ID: 84444
• mRNA Refseq: NM_032482
• Protein Refseq: NP_115871
• MIM: 607375
• Uniprot ID: Q8TEK3
• Chromosome Location: 19p13.3
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; Transcriptional misregulation in cancers, organism-specific biosystem; Transcriptional misregulation in cancers, conserved biosystem
• Function: DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; protein binding; transferase activity