Recombinant Human ECH1, His-tagged
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| Cat.No. : | ECH1-28224TH |
| Product Overview : | Recombinant full length Human ECH1, amino acids 34-328 with N terminal His tag; 316 amino acids with a predicted MWt 34.4 kDa including tag. |
| Description : | This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. |
| Protein length : | 295 amino acids |
| Conjugation : | HIS |
| Molecular Weight : | 34.400kDa inclusive of tags |
| Source : | E. coli |
| Form : | Liquid |
| Purity : | >90% by SDS-PAGE |
| Storage buffer : | Preservative: NoneConstituents: 10% Glycerol, 50mM Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0 |
| Storage : | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Sequences of amino acids : | MGSSHHHHHHSSGLV PRGSHMTGSSAQEAA SGVALGEAPDHSYES LRVTSAQKHVLHVQL NRPNKRNAMNKVFWR EMVECFNKISRDADC RAVVISGAGKMFTAG IDLMDMASDILQPKG DDVARISWYLRDIIT RYQETFNVIERCPKP VIAAVHGGCIGGGVD LVTACDIRYCAQDAF FQVKEVDVGLAADVG TLQRLPKVIGNQSLV NELAFTARKMMADEA LGSGLVSRVFPDKEV MLDAALALAAEISSK SPVAVQSTKVNLLYS RDHSVAESLNYVASW NMSMLQTQDLVKSVQ ATTENKELKTVTFSK L |
| Sequence Similarities : | Belongs to the enoyl-CoA hydratase/isomerase family. |
| Gene Name : | ECH1 enoyl CoA hydratase 1, peroxisomal [ Homo sapiens ] |
| Official Symbol : | ECH1 |
| Synonyms : | ECH1; enoyl CoA hydratase 1, peroxisomal; enoyl Coenzyme A hydratase 1, peroxisomal; delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; HPXEL; |
| Gene ID : | 1891 |
| mRNA Refseq : | NM_001398 |
| Protein Refseq : | NP_001389 |
| MIM : | 600696 |
| Uniprot ID : | Q13011 |
| Chromosome Location : | 19q13.1 |
| Pathway : | Fatty Acid Biosynthesis, organism-specific biosystem; Peroxisome, organism-specific biosystem; Peroxisome, conserved biosystem; |
| Function : | enoyl-CoA hydratase activity; isomerase activity; protein binding; |
◆ Recombinant Protein
| ECH1-3030H | Recombinant Human ECH1 Protein, GST-tagged | +Inquiry |
| ECH1-486H | Recombinant Human ECH1, His tagged | +Inquiry |
| ECH1-12267H | Recombinant Human ECH1, GST-tagged | +Inquiry |
| ECH1-4965M | Recombinant Mouse ECH1 Protein | +Inquiry |
| ECH1-1577Z | Recombinant Zebrafish ECH1 | +Inquiry |
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◆ Lysates
| ECH1-6730HCL | Recombinant Human ECH1 293 Cell Lysate | +Inquiry |
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