Recombinant Human ENSA protein, His-tagged
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|Product Overview :||Recombinant Human ENSA (Ser2-Glu121) protein was fused to His-tag at N-terminus and expressed in E.coli.|
|Description :||Alpha-endosulfine (ENSA),a member of the endosulfine family, is also known as ARPP-19e,which is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. ENSA is a protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, ENSA is able to specifically interact with PPP2R2D (PR55-delta) and inhibit its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). ENSA acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.|
|Predicted N Terminal :||Ser2|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 14.2 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).|
|Protein length :||Ser2-Glu121|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of ENSA-28569TH :||
Human ENSA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Gene Name :||ENSA|
|Official Symbol :||ENSA|
|Synonyms :||ENSA; endosulfine alpha; alpha-endosulfine; ARPP 19e; MGC4319; MGC8394; MGC78563; ARPP-19e|
|Gene ID :||2029|
|mRNA Refseq :||NM_004436|
|Protein Refseq :||NP_004427|
|UniProt ID :||O43768|
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