Recombinant Human FARS2, His-tagged

• Cat.No.: FARS2-28164TH
• Product Overview: Recombinant fragment of Human FARS2 with an N terminal His tag; 436 amino acids with the tag, predicted MWt: 50.6 kDa.

Specification

• Description: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm.
• Protein length: 415 amino acids
• Conjugation: HIS
• Molecular Weight: 50.600kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 30% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMPAAE CATQRAPGSVVELLGKSYPQDDHSN LTRKVLTRVGRNLHNQQHHPLWLIK ERVKEHFYKQYVGRFGTPLFSVYDN LSPVVTTWQNFDSLLIPADHPSRKK GDNYYLNRTHMLRAHTSAHQWDLLH AGLDAFLVVGDVYRRDQIDSQHYPI FHQLEAVRLFSKHELFAGIKDGESL QLFEQSSRSAHKQETHTMEAVKLVE FDLKQTLTRLMAHLFGDELEIRWVD CYFPFTHPSFEMEINFHGEWLEVLG CGVMEQQLVNSAGAQDRIGWAFGLG LERLAMILYDIPDIRLFWCEDERFL KQFCVSNINQKVKFQPLSKYPAVIN DISFWLPSENYAENDFYDLVRTIGG DLVEKVDLIDKFVHPKTHKTSHCYR ITYRHMERTLSQREVRHIHQALQEA AVQLLGVEGRF
• Sequence Similarities: Belongs to the class-II aminoacyl-tRNA synthetase family.Contains 1 FDX-ACB domain.

Gene Information

• Gene Name: FARS2 phenylalanyl-tRNA synthetase 2, mitochondrial [ Homo sapiens ]
• Official Symbol: FARS2
• Synonyms: FARS2; phenylalanyl-tRNA synthetase 2, mitochondrial; FARS1, phenylalanine tRNA synthetase 1 (mitochondrial); phenylalanyl-tRNA synthetase, mitochondrial; dJ236A3.1; phenylalanine tRNA ligase 2; mitochondrial
• Gene ID: 10667
• mRNA Refseq: NM_006567
• Protein Refseq: NP_006558
• MIM: 611592
• Uniprot ID: O95363
• Chromosome Location: 6p25.1
• Pathway: Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Gene Expression, organism-specific biosystem
• Function: ATP binding; ligase activity; magnesium ion binding; nucleotide binding; phenylalanine-tRNA ligase activity