Recombinant Human FARS2, His-tagged FARS2-28164TH

Recombinant Human FARS2, His-tagged

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Recombinant Human FARS2, His-tagged

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Cat.No. : FARS2-28164TH
Product Overview : Recombinant fragment of Human FARS2 with an N terminal His tag; 436 amino acids with the tag, predicted MWt: 50.6 kDa.
Description : Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm.
Protein length : 415 amino acids
Conjugation : HIS
Molecular Weight : 50.600kDa inclusive of tags
Source : E. coli
Form : Liquid
Purity : >90% by SDS-PAGE
Storage buffer : Preservative: NoneConstituents: 30% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 8.0
Storage : Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Sequences of amino acids : MGSSHHHHHHSSGLV PRGSHMPAAECATQR APGSVVELLGKSYPQ DDHSNLTRKVLTRVG RNLHNQQHHPLWLIK ERVKEHFYKQYVGRF GTPLFSVYDNLSPVV TTWQNFDSLLIPADH PSRKKGDNYYLNRTH MLRAHTSAHQWDLLH AGLDAFLVVGDVYRR DQIDSQHYPIFHQLE AVRLFSKHELFAGIK DGESLQLFEQSSRSA HKQETHTMEAVKLVE FDLKQTLTRLMAHLF GDELEIRWVDCYFPF THPSFEMEINFHGEW LEVLGCGVMEQQLVN SAGAQDRIGWAFGLG LERLAMILYDIPDIR LFWCEDERFLKQFCV SNINQKVKFQPLSKY PAVINDISFWLPSEN YAENDFYDLVRTIGG DLVEKVDLIDKFVHP KTHKTSHCYRITYRH MERTLSQREVRHIHQ ALQEAAVQLLGVEGR F
Sequence Similarities : Belongs to the class-II aminoacyl-tRNA synthetase family.Contains 1 FDX-ACB domain.
Gene Name : FARS2 phenylalanyl-tRNA synthetase 2, mitochondrial [ Homo sapiens ]
Official Symbol : FARS2
Synonyms : FARS2; phenylalanyl-tRNA synthetase 2, mitochondrial; FARS1, phenylalanine tRNA synthetase 1 (mitochondrial); phenylalanyl-tRNA synthetase, mitochondrial; dJ236A3.1; phenylalanine tRNA ligase 2; mitochondrial;
Gene ID : 10667
mRNA Refseq : NM_006567
Protein Refseq : NP_006558
MIM : 611592
Uniprot ID : O95363
Chromosome Location : 6p25.1
Pathway : Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Gene Expression, organism-specific biosystem;
Function : ATP binding; ligase activity; magnesium ion binding; nucleotide binding; phenylalanine-tRNA ligase activity;

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