Recombinant Human GPX4, His-tagged

• Cat.No.: GPX4-29076TH
• Product Overview: Recombinant full length Human Glutathione Peroxidase 4 , fused at the C terminus to a His tag; MWt: ~20 kDa by SDS Page.

Specification

• Description: Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.
• Protein length: 197 amino acids
• Conjugation: HIS
• Molecular Weight: 20.000kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Present primarily in testis.
• Form: Lyophilised:Reconstitute in an appropriate buffer.
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 50mM Tris HCl, 150mM Sodium chloride, 1mM DTT, pH 8.2
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Sequence Similarities: Belongs to the glutathione peroxidase family.

Gene Information

• Gene Name: GPX4 glutathione peroxidase 4 (phospholipid hydroperoxidase) [ Homo sapiens ]
• Official Symbol: GPX4
• Synonyms: GPX4; glutathione peroxidase 4 (phospholipid hydroperoxidase); phospholipid hydroperoxide glutathione peroxidase, mitochondrial; MCSP; PHGPx
• Gene ID: 2879
• mRNA Refseq: NM_001039847
• Protein Refseq: NP_001034936
• MIM: 138322
• Uniprot ID: P36969
• Chromosome Location: 19p13.3
• Pathway: Folate Metabolism, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; Glutathione metabolism, conserved biosystem; Selenium Metabolism and Selenoproteins, organism-specific biosystem
• Function: glutathione peroxidase activity; oxidoreductase activity; phospholipid-hydroperoxide glutathione peroxidase activity