Recombinant Human GTF2A2, His-tagged

• Cat.No.: GTF2A2-29171TH
• Product Overview: Recombinant full length Human GTF2A2 with His tag. Amino acids 1-109, predicted MW 12 kDa,

Specification

• Description: Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al.
• Conjugation: HIS
• Source: E. coli
• Form: Liquid
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.1mM EDTA, pH 7.9
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: GTF2A2 general transcription factor IIA, 2, 12kDa [ Homo sapiens ]
• Official Symbol: GTF2A2
• Synonyms: GTF2A2; general transcription factor IIA, 2, 12kDa; general transcription factor IIA, 2 (12kD subunit); transcription initiation factor IIA subunit 2; HsT18745; TFIIA
• Gene ID: 2958
• mRNA Refseq: NM_004492
• Protein Refseq: NP_004483
• MIM: 600519
• Uniprot ID: P52657
• Chromosome Location: 15q21.3
• Pathway: Basal transcription factors, organism-specific biosystem; Basal transcription factors, conserved biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Formation and Maturation of mRNA Transcript, organism-specific biosystem; Gene Expression, organism-specific biosystem
• Function: TBP-class protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription coactivator activity