Active Recombinant Human HDAC10 Protein, His-GST-tagged

• Cat.No.: HDAC10-27261TH
• Product Overview: Recombinant Human HDAC10 Protein (a.a. 1-481) is produced by Baculovirus Insect Cells expression system. This protein is fused with N-terminal GST-tag amd C-terminal His-tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: a.a. 1-481
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
• Bio-activity: Specific Activity: ≥4.4 pmol/min/µg. Assay Conditions: 25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3, and HDAC10 (15.6 - 2000 ng). Incubation condition: 30 min at 37 centigrade, followed by HDAC developer for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
• Molecular Mass: 78 kDa
• Purity: ≥19%
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Stability: >6 months at -80 centigrade.
• Storage: Store at -80 centigrade. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: HDAC10 histone deacetylase 10 [ Homo sapiens ]
• Official Symbol: HDAC10
• Synonyms: HDAC10; histone deacetylase 10; DKFZP761B039; HD10; MGC149722; DKFZp761B039
• Gene ID: 83933
• mRNA Refseq: NM_001159286
• Protein Refseq: NP_001152758
• MIM: 608544
• UniProt ID: Q969S8