||Recombinant Human follistatinexpressed inSf21 cells using a baculovirus expression system. And A DNA sequence encoding amino acid residues Gly 30 to Asp 329 of the human Follistatin precursor was fused to the signal peptide of human CD33, inserted into a suitable expression vector.
||Follistatin (FST) is a glycosylated single-chain protein. It specifically inhibits the secretion of FSH from the pituitary. Follistatin/activin-binding protein binds to heparan sulfate side chains of proteoglycans on the granulosa cell surface to regulate the various actions of activin. Follistatin regulates a variety of biological functions, including cell proliferation, differentiation, and apoptosis. Follistatin plays a critical role in the production of multiple-organ metastasis, predominantly by inhibiting the angiogenesis.
||Sf21 Insect Cells.
||The predicted molecular weight of Recombinant Human FS is 31 kDa. However, the actual molecular weight as observed by migration on SDS Page is 40 kDa.
|State Of Matter:
||>95% by SDS Page and analyzed by silver stain.
||<1.0 EU/µg as determined by the LAL method.
||The biological activity of Human FS was determined by its ability to neutralize Activin-induced bioactivity on K562 cells (erythroid differentiation). The expected ED50 for this effect is typically 0.1 - 0.4 μg/mL in the presence of 7.5 ng/mL of rhActivin A.