Recombinant Human Insulin-Like Growth Factor Binding Protein 5

• Cat.No.: IGFBP5-1629H
• Product Overview: Recombinant Human IGFBP-5 (Leu 21 - Glu 272) encoding the mature human IGFBP-5 protein sequence was fused to the signal peptide of CD33 and was inserted into a suitable expression vector. The chimeric protein was expressed in a mouse myeloma cell line,NSO.

Specification

• Cat. No.: IGFBP5-1629H
• Description: Insulin-like growth factor binding protein 5, also known as IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. It is expressed highly in the kidney. IGFBP-5 has an autonomous role in the regulation of cell fate in development and in tumourigenesis.
• Source: Mammalian cells
• State Of Matter: Lyophilized.
• Purity: >90% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Molecular Weight: The predicted molecular weight of Recombinant Human IGFBP-5 is Mr 28 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 34 kDa.
• Formulation: This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
• Biological Activity: The biological activity of Human IGFBP-5 was determined by by its ability to inhibit the biological activity of rhIGF-I or rhIGF-II on MCF-7 cells. The expected ED50 for this effect is typically 0.5 - 1.5 μg/mL in the presence of 14 ng/mL rhIGF-II.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Gene Information

• Gene Name: IGFBP5 insulin-like growth factor binding protein 5 [ Homo sapiens ]
• Synonyms: IBP-5; IBP5; IGFBP-5; IGFBP5; IGF-binding protein 5; insulin-like growth factor binding protein 5
• Gene ID: 3488
• mRNA Refseq: NM_000599
• Protein Refseq: NP_000590
• MIM: 146734
• UniProt ID: P24593
• Chromosome Location: 2q33-q36
• Pathway: Diabetes pathway
• Function: insulin-like growth factor I binding