Recombinant Human KARS, His-tagged

• Cat.No.: KARS-29008TH
• Product Overview: Recombinant fragment Human Lysyl tRNA synthetase (amino acids 63-597) with a N terminal His tag. 558 amino acids with a predicted MWt 63.7 kDa including tag.

Specification

• Description: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Protein length: 535 amino acids
• Conjugation: HIS
• Molecular Weight: 63.700kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride, 0.02% DTT
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMGSGV GPEEESVDPNQYYKIRSQAIHQLKV NGEDPYPHKFHVDISLTDFIQKYSH LQPGDHLTDITLKVAGRIHAKRASG GKLIFYDLRGEGVKLQVMANSRNYK SEEEFIHINNKLRRGDIIGVQGNPG KTKKGELSIIPYEITLLSPCLHMLP HLHFGLKDKETRYRQRYLDLILNDF VRQKFIIRSKIITYIRSFLDELGFL EIETPMMNIIPGGAVAKPFITYHNE LDMNLYMRIAPELYHKMLVVGGIDR VYEIGRQFRNEGIDLTHNPEFTTCE FYMAYADYHDLMEITEKMVSGMVKH ITGSYKVTYHPDGPEGQAYDVDFTP PFRRINMVEELEKALGMKLPETNLF ETEETRKILDDICVAKAVECPPPRT TARLLDKLVGEFLEVTCINPTFICD HPQIMSPLAKWHRSKEGLTERFELF VMKKEICNAYTELNDPMRQRQLFEE QAKAKAAGDDEAMFIDENFCTALEY GLPPTAGWGMGIDRVAMFLTDSNNI KEVLLFPAMKPEDKKENVATTDTLE STTVGTSV
• Sequence Similarities: Belongs to the class-II aminoacyl-tRNA synthetase family.

Gene Information

• Gene Name: KARS lysyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: KARS
• Synonyms: KARS; lysyl-tRNA synthetase; KARS2; lysine tRNA ligase
• Gene ID: 3735
• mRNA Refseq: NM_001130089
• Protein Refseq: NP_001123561
• MIM: 601421
• Uniprot ID: Q15046
• Chromosome Location: 16q23.1
• Pathway: Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Cytosolic tRNA aminoacylation, organism-specific biosystem
• Function: ATP binding; ligase activity; lysine-tRNA ligase activity; lysine-tRNA ligase activity; metal ion binding