Recombinant Human LCK
Cat.No. : | LCK-29937TH |
Product Overview : | Recombinant full length Human Lck with N terminal proprietary tag expressed by baculovirus in Sf9 insect cells, 84 kDa. |
- Specification
- Gene Information
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Description : | This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. |
Tissue specificity : | Expressed specifically in lymphoid cells. |
Biological activity : | Specific Activity: 1.96 pmol/min/μg. |
Form : | Liquid |
Purity : | >90% by SDS-PAGE |
Storage buffer : | Preservative: NoneConstituents: 30% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0 |
Storage : | Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles. |
Sequence Similarities : | Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.Contains 1 protein kinase domain.Contains 1 SH2 domain.Contains 1 SH3 domain. |
Gene Name : | LCK lymphocyte-specific protein tyrosine kinase [ Homo sapiens ] |
Official Symbol : | LCK |
Synonyms : | LCK; lymphocyte-specific protein tyrosine kinase; tyrosine-protein kinase Lck; |
Gene ID : | 3932 |
mRNA Refseq : | NM_001042771 |
Protein Refseq : | NP_001036236 |
MIM : | 153390 |
Uniprot ID : | P06239 |
Chromosome Location : | 1p34.3 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Alpha-synuclein signaling, organism-specific biosystem; Atypical NF-kappaB pathway, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CD28 co-stimulation, organism-specific biosystem; |
Function : | ATP binding; ATPase binding; CD4 receptor binding; CD8 receptor binding; SH2 domain binding; |
Products Types
◆ Recombinant Protein | ||
LCK-3021R | Recombinant Rat LCK Protein, His (Fc)-Avi-tagged | +Inquiry |
Lck-1300M | Recombinant Mouse Lck Protein, MYC/DDK-tagged | +Inquiry |
LCK-174H | Recombinant Human LCK Protein (SH3 Domain), GST tagged | +Inquiry |
LCK-273H | Recombinant Human LCK, MYC/DDK-tagged | +Inquiry |
LCK-3365R | Recombinant Rat LCK Protein | +Inquiry |
◆ Lysates | ||
LCK-681HCL | Recombinant Human LCK cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionLCK plays a vital role in the development and maturation of T cells in the thymus, especially during the processes of positive and negative selection, ensuring the generation of a functional and self-tolerant T-cell repertoire.
Yes, LCK activity can be modulated by several pharmacological agents, including kinase inhibitors like dasatinib, which have been explored for therapeutic purposes in various diseases.
LCK activation is regulated post-translationally through phosphorylation at specific tyrosine residues, with its activity being modulated by both activating and inhibitory phosphorylations.
LCK is a pivotal Src family kinase that plays a crucial role in initiating T-cell receptor (TCR) signaling by phosphorylating key tyrosines on the CD3 and ζ-chains of the TCR complex.
LCK interacts with other kinases such as ZAP-70 and Fyn in T-cell signaling pathways, orchestrating a cascade of phosphorylation events that lead to T-cell activation.
Dysregulation of LCK has been associated with various conditions, including autoimmune diseases, immunodeficiencies, and certain leukemias.
LCK contributes to TCR complex formation and activation by phosphorylating the immunoreceptor tyrosine-based activation motifs (ITAMs) on the TCR, facilitating the recruitment and activation of ZAP-70 kinase.
Customer Reviews (3)
Write a reviewGood stability, long shelf-life.
Consistent quality across batches.
Pure product, reliable results.
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