Recombinant Human Neurotrophin 3

• Cat.No.: NTF3-22H
• Product Overview: Recombinant Human Neurotrophin 3 encoding the NT-3 prepropeptide was inserted into a baculovirus expression vector and expressed inSf 21insect cells.

Specification

• Cat. No.: NTF3-22H
• Description: Neurotrophin 3 (NT-3), also known as NTF3 is a basic protein found in neurons of the central nervous system. NT-3 selectively supports the survival of neuronal cell populations. It rapidly potentiate the spontaneous and impulse-evoked synaptic activity of developing neuromuscular synapses in culture and thus appears to be involved in the regulation of functions of developing synapses. NT-3 negatively modulates nerve growth factor (NGF) receptor expression and associated nociceptive phenotype in intact neurons, suggesting a beneficial role in treating aspects of neuropathic pain mediated by NGF. NT-3 is effective at suppressing thermal hyperalgesia associated with chronic constriction injury (CCI).
• Molecular Weight: The predicted molecular weight of Recombinant Human NT-3 is Mr 13.6 kDa.
• Source: Sf 21 Insect Cells.
• State Of Matter: Lyophilized.
• Purity: >97% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human NT-3 was determined by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD. The expected ED50 for this effect is typically 10 - 30 ng/mL.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: NTF3 neurotrophin 3 [ Homo sapiens ]
• Synonyms: NTF3; neurotrophin 3; HDNF; NGF2; NGF-2; MGC129711; NT3; NT-3; Nerve growth factor 2; Neurotrophic factor
• Gene ID: 4908
• mRNA Refseq: NM_001102654
• Protein Refseq: NP_001096124
• MIM: 162660
• UniProt ID: P20783
• Chromosome Location: 12p13
• Pathway: Neurotrophin signaling pathway; MAPK signaling pathway
• Function: growth factor activity; nerve growth factor binding; neurotrophin receptor binding