||Recombinant Human Neurotrophin 3 encoding the NT-3 prepropeptide was inserted into a baculovirus expression vector and expressed inSf 21insect cells.
||Neurotrophin 3 (NT-3), also known as NTF3 is a basic protein found in neurons of the central nervous system. NT-3 selectively supports the survival of neuronal cell populations. It rapidly potentiate the spontaneous and impulse-evoked synaptic activity of developing neuromuscular synapses in culture and thus appears to be involved in the regulation of functions of developing synapses. NT-3 negatively modulates nerve growth factor (NGF) receptor expression and associated nociceptive phenotype in intact neurons, suggesting a beneficial role in treating aspects of neuropathic pain mediated by NGF. NT-3 is effective at suppressing thermal hyperalgesia associated with chronic constriction injury (CCI).
||The predicted molecular weight of Recombinant Human NT-3 is Mr 13.6 kDa.
||Sf 21 Insect Cells.
|State Of Matter:
||>97% by SDS Page and analyzed by silver stain.
||<1.0 EU/µg as determined by the LAL method.
||The biological activity of Human NT-3 was determined by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD. The expected ED50 for this effect is typically 10 - 30 ng/mL.
|Storage And Stability:
||This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.