Recombinant Human PHKG1

• Cat.No.: PHKG1-30332TH
• Product Overview: Recombinant full length Human PHKG1 expressed in Sf9 insect cells using an N terminal proprietary tag; predicted MWt: 70 kDa inclusive of tag

Specification

• Description: This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12.
• Protein length: 387 amino acids
• Molecular Weight: 70.000kDa inclusive of tags
• Source: Baculovirus
• Biological activity: The specific activity of PHKG1 was determined to be 29 nmol/min/mg.
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: MTRDEALPDSHSAQDFYENYEPKEI LGRGVSSVVRRCIHKPTSQEYAVKV IDVTGGGSFSPEEVRELREATLKEV DILRKVSGHPNIIQLKDTYETNTFF FLVFDLMKRGELFDYLTEKVTLSEK ETRKIMRALLEVICTLHKLNIVHRD LKPENILLDDNMNIKLTDFGFSCQL EPGERLREVCGTPSYLAPEIIECSM NEDHPGYGKEVDMWSTGVIMYTLLA GSPPFWHRKQMLMLRMIMSGNYQFG SPEWDDYSDTVKDLVSRFLVVQPQN RYTAEEALAHPFFQQYLVEEVRHFS PRGKFKVIALTVLASVRIYYQYRRV KPVTREIVIRDPYALRPLRRLIDAY AFRIYGHWVKKGQQQNRAALFENTP KAVLLSLAEEDY

Gene Information

• Gene Name: PHKG1 phosphorylase kinase, gamma 1 (muscle) [ Homo sapiens ]
• Official Symbol: PHKG1
• Synonyms: PHKG1; phosphorylase kinase, gamma 1 (muscle); PHKG; phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform
• Gene ID: 5260
• mRNA Refseq: NM_006213
• Protein Refseq: NP_006204
• MIM: 172470
• Uniprot ID: Q16816
• Chromosome Location: 7p11.2
• Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen breakdown (glycogenolysis), organism-specific biosystem
• Function: ATP binding; calmodulin binding; enzyme binding; nucleotide binding; phosphorylase kinase activity