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Recombinant Human PHKG1

Cat.No.: PHKG1-30332TH
Product Overview: Recombinant full length Human PHKG1 expressed in Sf9 insect cells using an N terminal proprietary tag; predicted MWt: 70 kDa inclusive of tag;
Description: This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12.
Protein length: 387 amino acids
Molecular Weight: 70.000kDa inclusive of tags
Source: Baculovirus
Biological activity: The specific activity of PHKG1 was determined to be 29 nmol/min/mg.
Form: Liquid
Purity: by SDS-PAGE
Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
Sequences of amino acids: MTRDEALPDSHSAQDFYENYEPKEILGRGVSSVVRRCIHKPTSQEYAVKVIDVTGGGSFSPEEVRELREATLKEVDILRKVSGHPNIIQLKDTYETNTFFFLVFDLMKRGELFDYLTEKVTLSEKETRKIMRALLEVICTLHKLNIVHRDLKPENILLDDNMNIKLTDFGFSCQLEPGERLREVCGTPSYLAPEIIECSMNEDHPGYGKEVDMWSTGVIMYTLLAGSPPFWHRKQMLMLRMIMSGNYQFGSPEWDDYSDTVKDLVSRFLVVQPQNRYTAEEALAHPFFQQYLVEEVRHFSPRGKFKVIALTVLASVRIYYQYRRVKPVTREIVIRDPYALRPLRRLIDAYAFRIYGHWVKKGQQQNRAALFENTPKAVLLSLAEEDY
Gene Name: PHKG1 phosphorylase kinase, gamma 1 (muscle) [ Homo sapiens ]
Official Symbol: PHKG1
Synonyms: PHKG1; phosphorylase kinase, gamma 1 (muscle); PHKG; phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform;
Gene ID: 5260
mRNA Refseq: NM_006213
Protein Refseq: NP_006204
MIM: 172470
Uniprot ID: Q16816
Chromosome Location: 7p11.2
Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycogen Metabolism, organism-specific biosystem; Glycogen breakdown (glycogenolysis), organism-specific biosystem;
Function: ATP binding; calmodulin binding; enzyme binding; nucleotide binding; phosphorylase kinase activity;

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