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Active Recombinant Human CERK, Flag-tagged

Cat.No. : CERK-508H
Product Overview : Recombinant Human CERK (1-537(end)), fused with C-terminal Flag tag, was expressed in baculovirus expression system.
  • Specification
  • Gene Information
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Description : CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation.
Source : Insect cells
Species : Human
Tag : Flag
Form : 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 40 ug/ml Flag peptide, 20% glycerol and 3 mM DTT.
Bio-activity : 142 pmole/min/μg. Assay conditions: CERK activity was measured at 37 oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide. ATP reduction was detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega, Madison, WI).
Molecular Mass : 60 kDa
Applications : Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Stability : >6 months at –80°C
Gene Name : CERK ceramide kinase [ Homo sapiens ]
Official Symbol : CERK
Synonyms : CERK; LK4; hCERK; dA59H18.2; dA59H18.3; ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase; NP_073603.2; EC 2.7.1.138
Gene ID : 64781
mRNA Refseq : NM_022766
Protein Refseq : NP_073603
MIM : 610307
UniProt ID : Q8TCT0
Chromosome Location : 22q13.31
Pathway : Glycosphingolipid metabolism; Integrated Breast Cancer Pathway; Sphingolipid Metabolism
Function : ATP binding; NAD+ kinase activity; ceramide kinase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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