Recombinant Human CERK, Flag-tagged CERK-508H

Recombinant Human CERK, Flag-tagged

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Recombinant Human CERK, Flag-tagged

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Cat.No. : CERK-508H
Product Overview : Recombinant Human CERK (1-537(end)), fused with C-terminal Flag tag, was expressed in baculovirus expression system.
Description : CERK converts ceramide to ceramide 1-phosphate (C1P), a sphingolipid metabolite. Both CERK and C1P have been implicated in various cellular processes, including proliferation, apoptosis, phagocytosis, and inflammation.
Source : Insect cells
Species : Human
Tag : Flag
Form : 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 40 ug/ml Flag peptide, 20% glycerol and 3 mM DTT.
Bio-activity : 142 pmole/min/μg. Assay conditions: CERK activity was measured at 37 oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide. ATP reduction was detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega, Madison, WI).
Molecular Mass : 60 kDa
Applications : Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Stability : >6 months at –80°C
Gene Name : CERK ceramide kinase [ Homo sapiens ]
Official Symbol : CERK
Synonyms : CERK; LK4; hCERK; dA59H18.2; dA59H18.3; ceramide kinase; lipid kinase 4; lipid kinase LK4; acylsphingosine kinase; NP_073603.2; EC 2.7.1.138
Gene ID : 64781
mRNA Refseq : NM_022766
Protein Refseq : NP_073603
MIM : 610307
UniProt ID : Q8TCT0
Chromosome Location : 22q13.31
Pathway : Glycosphingolipid metabolism; Integrated Breast Cancer Pathway; Sphingolipid Metabolism
Function : ATP binding; NAD+ kinase activity; ceramide kinase activity

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