Recombinant Human PSMB5, His-tagged

• Cat.No.: PSMB5-31226TH
• Product Overview: Recombinant full length Human PSMB5 with an N terminal His tag; 241 amino acids with tag, Predicted MWt 26.7 kDa.

Specification

• Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene.
• Protein length: 204 amino acids
• Conjugation: HIS
• Molecular Weight: 26.700kDa inclusive of tags
• Source: E. coli
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 0.2M Sodium chloride, 5mM DTT, 20mM Tris HCl, pH 8.0
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MRGSHHHHHHGMASMTGGQQMGRDL YDDDDKDRWGSMTTTLAFKFRHGVI VAADSRATAGAYIASQTVKKVIEIN PYLLGTMAGGAADCSFWERLLARQC RIYELRNKERISVAAASKLLANMVY QYKGMGLSMGTMICGWDKRGPGLYY VDSEGNRISGATFSVGSGSVYAYGV MDRGYSYDLEVEQAYDLARRAIYQA TYRDAYSGGAVNLYHVREDGWIRVS SDNVADLHEKYSGSTP
• Sequence Similarities: Belongs to the peptidase T1B family.

Gene Information

• Gene Name: PSMB5 proteasome (prosome, macropain) subunit, beta type, 5 [ Homo sapiens ]
• Official Symbol: PSMB5
• Synonyms: PSMB5; proteasome (prosome, macropain) subunit, beta type, 5; proteasome subunit beta type-5; MB1; X
• Gene ID: 5693
• mRNA Refseq: NM_001130725
• Protein Refseq: NP_001124197
• MIM: 600306
• Uniprot ID: P28074
• Chromosome Location: 14q11.2
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem
• Function: peptidase activity; protein binding; threonine-type endopeptidase activity