Active Recombinant Mouse Flt1 Protein, Fc-tagged

• Cat.No.: Flt1-1785M
• Product Overview: Recombinant mouse Flt1 protein (Ser27-Glu759) with mouse IgG2A (Glu98-Lys330) tag at C-terminus was expressed in Mouse myeloma cell line.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 27-759 a.a.
• Description: Biased expression in placenta adult (RPKM 35.7), lung adult (RPKM 11.8) and 11 other tissues.
• Form: Lyophilized
• Bio-activity: Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 5-30 ng/mL.
• Molecular Mass: 109.6 kDa (monomer)
• Endotoxin: <0.10 EU per 1 µg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 µm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 µg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.
• Publications:
  Syndecan-1 and glycosaminoglycans in pre-eclamptic and normotensive pregnancies. (2017)
  Vascular damage and dysfunction in hypertensive emergencies (2017)
  SFlt-1 Elevates Blood Pressure by Augmenting Endothelin-1-Mediated Vasoconstriction in Mice (2014)
  Soluble syndecan-1 and glycosaminoglycans in preeclamptic and normotensive pregnancies (2021)

Gene Information

• Gene Name: Flt1 FMS-like tyrosine kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Flt1
• Synonyms: Flt1; FMS-like tyrosine kinase 1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757; vascular endothelial growth factor receptor 1; embryonic receptor kinase 2; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; EC 2.7.10.1
• Gene ID: 14254
• mRNA Refseq: NM_010228
• Protein Refseq: NP_034358
• UniProt ID: P35969

Citation

SFlt-1 Elevates Blood Pressure by Augmenting Endothelin-1-Mediated Vasoconstriction in Mice

Journal: PLoS ONE    PubMed ID: 24632840    Data: 2022/12/14

Authors: Fouad Amraoui, Léon Spijkers, Christopher Torrens

Article Snippet:A single dose of buprenorphine (0.05 mg/kg s.c., Schering-Plough) was administered for postoperative analgesia.A single dose of buprenorphine (0.05 mg/kg s.c., Schering-Plough) was administered for postoperative analgesia.. The osmotic minipumps were filled with either vehicle (phosphate-buffered saline, PBS) or recombinant mouse sFlt-1 (Creative BioMart, catalog no: Flt1-1785M) for continuous 0.5μl/h compound release (equals 500 ng/h sFlt-1) during 2 weeks.. Aspirin (30 mg/kg/day Cayman Chemical) or picotamide (5 mg/kg/day, Sigma) dissolved in minimal amounts of EtOH (<0.1%) were added to the drinking water during sFlt-1 treatment in a subset of mice.Aspirin (30 mg/kg/day Cayman Chemical) or picotamide (5 mg/kg/day, Sigma) dissolved in minimal amounts of EtOH (<0.1%) were added to the drinking water during sFlt-1 treatment in a subset of mice.

In vivo effect of sFlt-1 or vehicle (Cntrl) infusion during two weeks on mean arterial pressure (MAP). Data are presented as mean±SEM, N = 11–12, (ns) not significant, * p <0.05.

A) Concentration-response curve of endothelin-1 in isolated carotid arteries of vehicle-treated (Cntrl) and sFlt-1-treated mice. The inset depicts concentration-response curves of KCl (N = 15) and the α1-adrenergic receptor agonist phenylephrine (N = 8–9). B ) Effect of pre-incubation with the non-selective cyclooxygenase-inhibitor indomethacin (indo; 10 μmol/L) on endothelin-1 concentration-response curves. Data are expressed as mean±SEM, *(maximal efficacy of sFlt-1 vs. Cntrl, N = 9–10, p <0.05), #(maximal efficacy of sFlt-1 vs. sFlt-1 + indo, N = 9–10, p <0.05), $(EC 50 of sFlt-1 vs. sFlt-1 + indo and Cntrl vs. Cntrl + indo, p <0.05)

A) Effect of eNOS inhibition with L-NAME (N = 5) and endothelium (EC) denudation (N = 4) on ET-1 concentration-response curves in carotid segments of vehicle-treated mice (Cntrl). Data are expressed as mean±SEM, *(maximal efficacy of Cntrl vs. Cntrl + L-NAME and Cntrl vs. Cntrl + EC denuded, N = 9–5, p <0.05). B ) Effect of L-NAME (N = 4) and EC denudation (N = 3) on ET-1 concentration-response curves in carotid segments of sFlt-1-treated mice. C ) Metacholine concentration-response curve generated after pre-constriction with the α1-adrenergic receptor agonist phenylephrine. Data are expressed as mean±SEM,*(maximal relaxation sFlt-1 vs. Cntrl, N = 13–14, p <0.05).