|Product Overview:||Recombinant Mouse Flt1(Ser27-Glu759) fused with His tag at C-terminal was expressed in NS0.|
|Predicted N Terminal:||Ser27|
|Form:||Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CHAPS.|
|Bio-activity:||Measured by its ability to inhibit the VEGF-dependent proliferation of HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 10~30 ng/mL.|
|Molecular Mass:||Predicted Molecular Mass: 110 kDa (monomer)
SDS-PAGE: 150-170 kDa, reducing conditions
|Endotoxin:||<0.10 EU per 1 μg of the protein by the LAL method.|
|Purity:||>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Gene Name:||Flt1 FMS-like tyrosine kinase 1 [ Mus musculus ]|
|Synonyms:||FLT1; FMS-like tyrosine kinase 1; vascular endothelial growth factor receptor 1; embryonic receptor kinase 2; tyrosine-protein kinase receptor FLT; vascular permeability factor receptor; vascular endothelial growth factor receptor-1; Flt-1; sFlt1; VEGFR1; VEGFR-1; AI323757;|