| Description : |
Plasminogen activator inhibitor-2 (placental PAI, SerpinB2, PAI-2), a serine protease inhibitor of the serpin superfamily, is a coagulation factor that inactivates tPA and urokinase. It is present in most cells, especially monocytes/macrophages. PAI-2 exists in two forms, a 60-kDa extracellular glycosylated form and a 43-kDa intracellular form. |
| Source : |
E.coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM Cysteine, with 5 % Trehalose. |
| Bio-activity : |
Fully biologically active when compared to standard. The biologically active was determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37 centigrade. Half maximal inhibition against 1.0 μg/ml of single chain tPA was at a concentration of 1.0 μg/ml. |
| Molecular Mass : |
Approximately 46.6 kDa, a single non-glycosylated polypeptide chain containing 415 amino acids. |
| Protein length : |
415 |
| AA Sequence : |
MEDLCVANTLFALNLFKHLAKASPT QNLFLSPWSISSTMAMVYMGSRGST EDQMAKVLQFNEVGANAVTPMTPEN FTSCGFMQQIQKGSYPDAILQAQAA DKIHSSFRSLSSAINASTGNYLLES VNKLFGEKSASFREEYIRLCQKYYS SEPQAVDFLECAEEARKKINSWVKT QTKGKIPNLLPEGSVDGDTRMVLVN AVYFKGKWKTPFEKKLNGLYPFRVN SAQRTPVQMMYLREKLNIGYIEDLK AQILELPYAGDVSMFLLLPDEIADV STGLELLESEITYDKLNKWTSKDKM AEDEVEVYIPQFKLEEHYELRSILR SMGMEDAFNKGRANFSGMSERNDLF LSEVFHQAMVDVNEEGTEAAAGTGG VMTGRTGHGGPQFVADHPFLFLIMH KITNCILFFGRFSSP |
| Endotoxin : |
Less than 0.1 EU/µg of rHuPAI-2 as determined by LAL method. |
| Purity : |
>95% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
