||Recombinant fragment: 110-476Human ACK1 with N terminal proprietary tag expressed in a Baculovirus infected Sf9 cell expression system, 68 kDa.
||This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
||Specific Activity: 88 pmol/min/mg.
||Preservative: NoneConstituents: 20% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8
||Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
|Sequences of amino acids: