Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human TOP1, His-tagged

Cat.No. : TOP1-30912TH
Product Overview : DNA Topoisomerase-I Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 Dalton (excluding glycosilation) 102 kDa total mass. The TOP1 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
Unit
Price
Qty
  • Specification
  • Gene Information
  • Related Products
Description : This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Source : Sf9 insect cells.
Species : Human
Tag : His
Form : Sterile Filtered clear solution. TOP1 is supplied in 16mM HEPES buffer pH-7.5, 400mM sodium chloride, and 20% glycerol.
Purity : Greater than 95% as determined by SDS-PAGE.
Storage : Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Publication :
Suppression of lupus nephritis and skin lesions in MRL/lpr mice by administration of the topoisomerase I inhibitor irinotecan (2016)
The topoisomerase I inhibitor irinotecan and the tyrosyl‐DNA phosphodiesterase 1 inhibitor furamidine synergistically suppress murine lupus nephritis (2015)
Low dose irinotecan improves advanced lupus nephritis in mice potentially by changing DNA relaxation and anti‐dsDNA binding. (2014)
Gene Name : TOP1 topoisomerase (DNA) I [ Homo sapiens (human) ]
Official Symbol : TOP1
Synonyms : TOP1; TOPI; topoisomerase (DNA) I; DNA topoisomerase 1; type I DNA topoisomerase; NP_003277.1; EC 5.99.1.2
Gene ID : 7150
mRNA Refseq : NM_003286
Protein Refseq : NP_003277
MIM : 126420
UniProt ID : P11387
Chromosome Location : 20q12-q13.1
Pathway : Caspase cascade in apoptosis; Integrated Pancreatic Cancer Pathway
Function : ATP binding; DNA topoisomerase type I activity; DNA topoisomerase type II (ATP-hydrolyzing) activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
What is the role of TOP1 in the repair of DNA damage caused by environmental factors and chemotherapeutic agents? 12/01/2021

TOP1 repairs DNA damage, maintaining genomic stability under stress.

How do post-translational modifications of TOP1, such as phosphorylation, affect its enzymatic activity and interaction with DNA? 07/17/2021

Post-translational modifications like phosphorylation affect TOP1's activity and DNA interaction.

How do mutations in the TOP1 gene contribute to genomic instability and the development of certain cancers? 02/28/2021

Mutations in TOP1 cause genomic instability, increasing cancer risk.

What are the effects of TOP1 inhibitors on cancer cell proliferation and survival? 12/12/2019

TOP1 inhibitors lead to cancer cell death and inhibit tumor growth by disrupting DNA processes.

How does TOP1 interact with other proteins in the DNA repair and replication machinery? 09/22/2019

TOP1 works with other DNA repair proteins, ensuring accurate DNA synthesis.

How does TOP1 regulate DNA unwinding and re-ligation during transcription and replication? 09/20/2019

TOP1 regulates DNA unwinding by creating transient breaks, aiding transcription and replication.

What are the implications of TOP1 expression levels in predicting the response to chemotherapy in cancer patients? 09/17/2018

TOP1 expression levels predict chemotherapy responses in cancer treatment.

Customer Reviews (3)

Write a review
Reviews
10/17/2019

    Accurate protein structural analysis; a key for structural biology.

    10/06/2018

      High-quality protein crystallography; essential for structural biology.

      05/30/2018

        Timely protein characterization; vital for our research projects.

        Ask a Question for All TOP1 Products

        Required fields are marked with *

        My Review for All TOP1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends