Recombinant Human XIAP, FLAG-tagged

• Cat.No.: XIAP-30701TH
• Product Overview: Recombinant full length Human XIAP with N terminal FLAG tag; MWt 57kDa.

Specification

• Description: This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.
• Protein length: 496 amino acids
• Conjugation: FLAG
• Molecular Weight: 57.000kDa inclusive of tags
• Source: Baculovirus
• Tissue specificity: Ubiquitous, except peripheral blood leukocytes.
• Biological activity: Specific Activity: 0.0015 pmol/min/μgAssay conditions: 4 mM ATP, 5mM MgCl2 (both required for reaction), 1mM DTT, E1 (0.5 μg), E2 (2.8 μg), Biotinylated Ubiquitin (1 μg) in 50mM Tris pH 7.4.
• Form: Liquid
• Storage buffer: pH: 8.00Constituents:20% Glycerol, 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Tween, 0.0462% DTTNote: 104 μg/ml FLAG peptide
• Storage: Store at -80°C
• Sequence Similarities: Belongs to the IAP family.Contains 3 BIR repeats.Contains 1 RING-type zinc finger.

Gene Information

• Gene Name: XIAP X-linked inhibitor of apoptosis [ Homo sapiens ]
• Official Symbol: XIAP
• Synonyms: XIAP; X-linked inhibitor of apoptosis; API3, baculoviral IAP repeat containing 4 , BIRC4; baculoviral IAP repeat-containing protein 4; hILP
• Gene ID: 331
• mRNA Refseq: NM_001167
• Protein Refseq: NP_001158
• MIM: 300079
• Uniprot ID: P98170
• Chromosome Location: Xq25
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptotic factor-mediated response, organism-specific biosystem
• Function: cysteine-type endopeptidase inhibitor activity involved in apoptotic process; ligase activity; metal ion binding; peptidase inhibitor activity; protein binding