Cat. No. : |
Wnt3a-1788M |
Description : |
Wingless-type MMTV integration site family, member 3A, also known as WNT3A is an intercellular signaling molecule. WNT3A is expressed extensively in cells fated to give rise to embryonic mesoderm. Wnt-3a regulates survival, expansion, and maintenance of neural progenitors derived from embryonic stem cells. It has been reported that Wnt-3a supports self-renewal of embryonic stem cells and can maintain these cells in a pluripotent state. Wnt-3a is also considered a ligand that activates the beta-catenin-dependent pathway in Wnt signaling, which is implicated in numerous physiological events such as morphogenesis. |
Molecular Weight : |
The predicted molecular weight of Recombinant Mouse WNT-3a is Mr 37 kDa. However, because of glycosylation the actual molecular weight as observed by migration on SDS Page is Mr 41 kDa. |
Source : |
CHO. |
State Of Matter : |
Lyophilized. |
Purity : |
>75% as determined by SDS-PAGE and visualized by silver stain. |
Endotoxin : |
<1.0 EU/µg as determined by the LAL method. |
Biological Activity : |
The biological activity of Mouse WNT-3a was determined by its ability to synergize with BMP-2 to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells. The expected ED50 is typically 2 - 10 ng/mL, in the presence of a low or non-inducing amount of recombinant human BMP-2 (5 - 15 ng/mL). Approximately 50 - 100 ng/mL results in maximal synergy with BMP-2 activity. |
Storage And Stability : |
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. |