Recombinant Human Enolase 1, (Alpha)

• Cat.No.: ENO1-1814H
• Product Overview: Recombinant human alpha-enolase was expressed inE.coliand purified by using conventional chromatography. MW = 47.1 kDa (434aa).

Specification

• Cat. No.: ENO1-1814H
• Description: Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto"s encephalopathy and severe asthma.
• Source: E.coli.
• Amino Acid Sequence: MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM MgSO4, 10% Glycerol.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publication:
  Miogènesi i regeneració muscular: paper d'alfa-enolasa/plasminogen i receptor del factor del creixement epitelial (2015)

Gene Information

• Gene Name: ENO1 enolase 1, (alpha) [ Homo sapiens ]
• Synonyms: ENO1; enolase 1, (alpha); NNE; PPH; MPB1; MBP-1; ENO1L1; enolase 1; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase; EC 4.2.1.11; MBPB1; OTTHUMP00000001706; C-myc promoter-binding protein; Plasminogen-binding protein; Alpha-enolase
• Gene ID: 2023
• mRNA Refseq: NM_001428
• Protein Refseq: NP_001419
• MIM: 172430
• UniProt ID: P06733
• Chromosome Location: 1p36.3-p36.2
• Pathway: Glycolysis / Gluconeogenesis; Metabolic pathways; RNA degradation
• Function: lyase activity; magnesium ion binding; phosphopyruvate hydratase activity; protein binding; serine-type endopeptidase activity; transcription corepressor activity; transcription factor activity