||Recombinant human Ghrelin protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 12.8 kDa (115aa).
||Ghrelin, also known as Obestatin, is the ligand for growth hormone secretagogue receptor type 1 (GHSR). It induces the release of growth hormone from the pituitary and involved in growth regulation. This protein has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Ghrelin plays a significant role in neurotrophy, particularly in the hippocampus, and is essential for cognitive adaptation to changing environments and the process of learning.
|Amino Acid Sequence:
||MGSSHHHHHH SSGLVPRGSH MGSSFLSPEH QRVQQRKESK KPPAKLQPRA LAGWLRPEDG GQAEGAEDEM EVRFNAPFDV GIKLSGVQYQ QHSQALGKFL QDILWEEAKE APADK
||Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
||> 90% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
||< 1.0 EU per 1 μg of protein (determined by LAL method).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.