||Recombinant Zea Mays Casein Kinase 2 alpha is a non-glycosylated polypeptide having a molecular mass of 39.2 kDa. Casein Kinase 2 is purified by proprietary chromatographic techniques. It is produced inE.Coli.
||The Casein kinase 2 (EC188.8.131.52) is a serine/threonine-selective protein kinasethat is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cyclecontrol, DNA repair, regulation of the circadian rhythmand other cellular processes. Casein kinase 2 activity has been reported to be activated following Wnt signaling pathwayactivation. A Pertussis toxin-sensitive G proteinand Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2.
||CK2a is supplied in 50% glycerol.
||Greater than 99% as determined by SDS-PAGE.
||No protease activity detectable, specific activity > 1U/mg (1U = 1µmol/min at 37°C) using the synthetic peptide RRRDDDSDDD.
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.