||Recombinant human Glutaredoxin 2, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.
||Glutaredoxin-2, as known as GRX2, multifunctional enzyme with glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase(GST) activity. This protein is localized both in the mitochondria and nucleus. It has anti-apoptotic activity by preventing cardiolipin oxidation and cytochrome c release. GRX2 exhibited 36% identity with GRX1 and had a disulfide active center containing the Cys-Ser-Tyr-Cys motif.
|Sequences of amino acids:
||MSAGWLDRAAGAAGAAAAAASGMESNTSSSLENLATAPVNQIQETISDNC VVIFSK TSCSYCTMAKKLFHDMNVNYKVVELDLLEYGNQFQDALYKMTGERTVPRIFVNGTF I GGATDTH RLHKEGKLLP LVHQCYLKKS KRKEFQLEHH HHHH
||Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1 mM PMSF, 10% Glycerol
||17.0 kDa (154aa)
||> 90% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.