||Syn61-140 was overexpressed inE. coliand was purified to apparent homogeneity by taking advantage of the thermosolubility of protein and by using conventional column chromatography techniques (81 aa, 8.4 kDa). Additional amino acid(Met) is attached at the N-terminus.
||A deletion mutant ofa-synuclein (amino acids 61-140). Alpha-synuclein also known as SNCA is a protein which in humans is encoded by the SNCA gene. An alpha-synuclein fragment, known as the non-Abeta component (NAC) of Alzheimer"s disease amyloid, originally found in an amyloid-enriched fraction, is shown to be a fragment of its precursor protein, NACP, by cloning of the full-length cDNA. Later, NACP turned out to be a human homologue of Torpedo synuclein. Therefore, NACP is also referred to as human alpha-synuclein.
||Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl.
||8.4 kDa (81 aa).
||> 95% by SDS – PAGE.
||1 mg/ml (determined by BCA assay).
|Amino acid Sequence:
||MEQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.