||Recombinant ANXA4 protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 36 kDa (321aa).
||ANXA4 belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. Isolated from human placenta, this protein has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANXA4 is almost exclusively expressed in epithelial cells.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.2M NaCl.
||> 90% by SDS – PAGE.
||1 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MAMATKGGTVKAASGFNAMEDAQTLRKAMKGLGTDEDAIISVLAYRNTAQ RQEIR TAYKSTIGRDLIDDLKSELSGNFEQ VIVGMMTPTVLYDVQELRRA MKGAGTDEGC LIEILASRTPEEIRRISQTYQQQYGRSLEDDIRSDTSFMFQRVLVSLSAG GRDEGN Y LDDALVRQDAQDLYEAGEKKWGTDEVKFLTVLCSRNRNHLLHVFDEYKRISQK DIE QSIKSETSGSFEDALLA IVKCMRNKSA YFAEKLYKSM KGLGTDDNTLIRVMVSRAEI DMLDIRAHFK RLYGKSLYSF IKGDTSGDYR KVLLVLCGGD D.
||can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.