Active Recombinant Human ALDH3A1 protein, His-tagged

• Cat.No.: ALDH3A1-497H
• Product Overview: Recombinant Human ALDH3A1(1-453aa) fused with His tag was expressed in E. coli, Bioactivity Validated.

Specification

• Description: ALDH3A1, also known as Aldehyde dehydrogenase 3 family memberA1, is involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to uV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. Recombinant ALDH3A1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl
• Bio-activity: Specific activity is > 5,000 pmol/min/ug. It was obtained by measuring the increase of NADPH in absorbance at 340nm resurting from the reduction of NADP at pH9.0 at 37C.
• Molecular Mass: 52.5 kDa (473aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSKISEAVKR ARAAFSSGRT RPLQFRIQQL EALQRLIQEQ EQELVGALAA DLHKNEWNAY YEEVVYVLEE IEYMIQKLPE WAADEPVEKT PQTQQDELYI HSEPLGVVLV IGTWNYPFNL TIQPMVGAIA AGNAVVLKPS ELSENMASLL ATIIPQYLDK DLYPVINGGV PETTELLKER FDHILYTGST GVGKIIMTAA AKHLTPVTLE LGGKSPCYVD KNCDLDVACR RIAWGKFMNS GQTCVAPDYI LCDPSIQNQI VEKLKKSLKE FYGEDAKKSR DYGRIISARH FQRVMGLIEG QKVAYGGTGD AATRYIAPTI LTDVDPQSPV MQEEIFGPVLPIVCVRSLEE AIQFINQREK PLALYMFSSN DKVIKKMIAE TSSGGVAAND VIVHITLHSL PFGGVGNSGM GSYHGKKSFE TFSHRRSCLV RPLMNDEGLK VRYPPSPAKM TQH
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ALDH3A1 aldehyde dehydrogenase 3 family, member A1 [ Homo sapiens ]
• Official Symbol: ALDH3A1
• Synonyms: ALDH3A1; aldehyde dehydrogenase 3 family, member A1; ALDH3; aldehyde dehydrogenase, dimeric NADP-preferring; aldehyde dehydrogenase; dimeric NADP preferring; stomach aldehyde dehydrogenase; aldehyde dehydrogenase type III; aldehyde dehydrogenase isozyme 3; ALDHIII; MGC10406
• Gene ID: 218
• mRNA Refseq: NM_000691
• Protein Refseq: NP_000682
• MIM: 100660
• UniProt ID: P30838
• Chromosome Location: 17p11.2
• Pathway: Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem; Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem; Histidine metabolism, organism-specific biosystem; Histidine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem
• Function: 3-chloroallyl aldehyde dehydrogenase activity; alcohol dehydrogenase (NADP+) activity; aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; oxidoreductase activity