||Recombinant human BAS protein (a.a. 2091-2490) with N-terminus GST-tag was expressed inE.coliexpression system, MW=71.8 kDa.
||BAS gene locateson chromosome 21 and participates in regulating the fl-adrenergic response of human fibroblasts.
||25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
||> 80% by SDS-PAGE.
||5 U/µg. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, and 100 mM NaCl. The specific activity of Bas was determined using pNPP. Enzyme reaction condition: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mMDTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme, for 5 min at 30°C.
||Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
||>6 months at –80°C.