| Species : |
Human |
| Source : |
E.coli |
| Tag : |
GST |
| Protein Length : |
2091-2490 a.a. |
| Description : |
BAS gene locateson chromosome 21 and participates in regulating the fl-adrenergic response of human fibroblasts. |
| Formulation : |
25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione. |
| Purity : |
> 80% by SDS-PAGE. |
| Specific Activity : |
5 U/µg. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, and 100 mM NaCl. The specific activity of Bas was determined using pNPP. Enzyme reaction condition: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mMDTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme, for 5 min at 30°C. |
| Application : |
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. |
| Storage : |
>6 months at –80°C. |
