Recombinant Human BAS, GST-tagged BAS-1855H

Cat. No. :	BAS-1855H
Product Overview :	Recombinant human BAS protein (a.a. 2091-2490) with N-terminus GST-tag was expressed inE.coliexpression system, MW=71.8 kDa.
Description :	BAS gene locateson chromosome 21 and participates in regulating the fl-adrenergic response of human fibroblasts.
Source :	E.coli.
Formulation :	25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Purity :	> 80% by SDS-PAGE.
Specific Activity :	5 U/µg. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, and 100 mM NaCl. The specific activity of Bas was determined using pNPP. Enzyme reaction condition: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mMDTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme, for 5 min at 30°C.
Application :	Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Storage :	>6 months at –80°C.

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Gene Name :	BAS Beta-adrenergic stimulation, response to [ Homo sapiens ]
Synonyms :	BAS; Beta-adrenergic stimulation, response to
Gene ID :	8213
MIM :	109670
Chromosome Location :	21

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