Recombinant Human BAS, GST-tagged

• Cat.No.: BAS-1855H
• Product Overview: Recombinant human BAS protein (a.a. 2091-2490) with N-terminus GST-tag was expressed inE.coliexpression system, MW=71.8 kDa.

Specification

• Cat. No.: BAS-1855H
• Description: BAS gene locateson chromosome 21 and participates in regulating the fl-adrenergic response of human fibroblasts.
• Source: E.coli.
• Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
• Purity: > 80% by SDS-PAGE.
• Specific Activity: 5 U/µg. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, and 100 mM NaCl. The specific activity of Bas was determined using pNPP. Enzyme reaction condition: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mMDTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme, for 5 min at 30°C.
• Application: Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
• Storage: >6 months at –80°C.

Gene Information

• Gene Name: BAS Beta-adrenergic stimulation, response to [ Homo sapiens ]
• Synonyms: BAS; Beta-adrenergic stimulation, response to
• Gene ID: 8213
• MIM: 109670
• Chromosome Location: 21