| Cat. No.: | BAS-1855H |
| Product Overview: | Recombinant human BAS protein (a.a. 2091-2490) with N-terminus GST-tag was expressed inE.coliexpression system, MW=71.8 kDa. |
| Description: | BAS gene locateson chromosome 21 and participates in regulating the fl-adrenergic response of human fibroblasts. |
| Source: | E.coli. |
| Formulation: | 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione. |
| Purity: | > 80% by SDS-PAGE. |
| Specific Activity: | 5 U/µg. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, and 100 mM NaCl. The specific activity of Bas was determined using pNPP. Enzyme reaction condition: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3mMDTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme, for 5 min at 30°C. |
| Application: | Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. |
| Storage: | >6 months at –80°C. |
| Gene Name: | BAS Beta-adrenergic stimulation, response to [ Homo sapiens ] |
| Synonyms: | BAS; Beta-adrenergic stimulation, response to |
| Gene ID: | 8213 |
| MIM: | 109670 |
| Chromosome Location: | 21 |
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