Recombinant Human BTK, GST-tagged

• Cat.No.: BTK-530H
• Product Overview: Recombinant full-length human BTK was expressed by baculovirus inSf9 insect cellsusing a N-terminal GST tag. MW=106kDa.

Specification

• Cat. No.: BTK-530H
• Description: BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation. BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors. Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. The unique role of BTK makes it a desirable target for potential anti-cancer, anti-inflammatory and anti-viral agents as well as other treatments.
• Source: Sf9 insect cells using baculovirus.
• Purity: >80%.
• Specific Activity: 23 pmol/min/μg.
• Assay condition: The enzyme reaction wascarried out for 1h at room temperature in abuffer containing 50 mM HEPES (pH7.5),10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 μM of ATP. Substrate : 2 μM Tyr peptide 1 from Invitrogen.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Stability: >6 months at –80°C.

Gene Information

• Gene Name: BTK Bruton agammaglobulinemia tyrosine kinase [ Homo sapiens ]
• Synonyms: BTK; Bruton agammaglobulinemia tyrosine kinase; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1; MGC126261; MGC126262; OTTHUMP00000023676; OTTHUMP00000063593; Bruton tyrosine kinase; B-cell progenitor kinase; tyrosine-protein kinase BTK; agammaglobulinemia tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase
• Gene ID: 695
• mRNA Refseq: NM_000061
• Protein Refseq: NP_000052
• MIM: 300300
• UniProt ID: Q06187
• Chromosome Location: Xq21.33-q22
• Pathway: B cell receptor signaling pathway; Fc epsilon RI signaling pathway
• Function: ATP binding; identical protein binding; kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein tyrosine kinase activity; transferase activity; zinc ion binding

Reviews

02/08/2022

High purity, lack of impurities, in the experiment performance is very efficient.

02/03/2022

Short half-life, more flexible dosage plan.

09/12/2021

It's stable in all kinds of environments.

Q&As

What is the current progress in drug development for BTK?

The development of drugs targeting BTK is currently in the clinical trial stage, including small molecule inhibitors against BTK and inhibitors targeting BTK signaling pathways.

How can BTK be used as a biomarker in clinical practice?

In clinical practice, the expression level of BTK can be detected to predict disease progression and prognosis, and to guide the choice of treatment regimen. For example, in the diagnosis and treatment of lymphoma and leukemia, testing the expression level of BTK can help doctors determine the type, stage and prognosis of the disease, and guide the choice of treatment regimen.

What is the significance of studying BTK proteins?

BTK proteins can help to gain insight into the mechanisms of B cell development and activation, as well as the pathogenesis of certain diseases such as lymphoma and leukemia. In addition, it is of great significance for the development of new drugs and treatment strategies.

Does BTK protein play a role in tumor immunotherapy?

BTK protein plays an important role in tumor immunotherapy, which can activate the immune response of tumor cells, promote apoptosis of tumor cells and inhibit the proliferation of tumor cells.

How is BTK protein related to viral infection?

Studies have shown that BTK protein can participate in the regulation of antiviral immune responses and affect the course of viral infection.

What are the applications of BTK protein in drug discovery?

It can be used as a target for the development of new drugs, or as a drug carrier for the directional delivery of drugs.